Click image to see more details
Product Info Summary
| SKU: | FEK1225 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
Customers Who Bought This Also Bought
How Quick Picokine(R) ELISA kits work: Get data in 90 minutes
Product info
Product Name
Mouse CCL9/Mip 1 Gamma PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1225
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CCL9/Mip 1 Gamma PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Ccl9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Mouse CCL9/Mip 1 Gamma PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1225)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Q22-Q122
Sensitivity
<1 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
See datasheet of FEK1225 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1225 is reactive to Ccl9 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK1225 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Ccl9
Chemokine (C-C motif) ligand 9 (CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium (FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1 (the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1225-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1225-ST | Mouse Ccl9 Standard | 2 | 10 ng/tube |
| FEK1225-A | Mouse Ccl9 Antibody A | 1 | 3ml |
| FEK1225-B | Mouse Ccl9 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.064 | 0.138 | 0.189 | 0.295 | 0.476 | 0.821 | 1.396 | 2.183 |
Data Example Images
Click image to see more details
Mouse CCL9 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CCL9/Mip 1 Gamma PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 12 | 77 | 272 | 12 | 74 | 290 |
| Standard deviation | 0.86 | 4.69 | 19.04 | 0.9 | 4.88 | 22.33 |
| CV (%) | 7.2% | 6.1% | 7% | 7.5% | 6.6% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Ccl9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 12 | 12 | 12 | 11 | 11 | 0.43 | 3.9% |
| Sample 2 | 77 | 67 | 78 | 82 | 76 | 5.52 | 7.2% |
| Sample 3 | 272 | 286 | 279 | 302 | 284 | 11.12 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For Ccl9 (Source: Uniprot.Org, NCBI)
Gene Name
Ccl9
Full Name
C-C motif chemokine 9
Weight
13871 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 9;CCF18;Macrophage inflammatory protein 1-gamma;MIP-1-gamma;Macrophage inflammatory protein-related protein 2;MRP-2;Small-inducible cytokine A9;CCL9 (29-101);CCL9 (30-101);CCL9 (31-101);Ccl9;Mrp2, Scya10, Scya9; Ccl9|CCF18, MRP, MRP-2, Scy, Scya, Scya10, Scya9|chemokine (C-C motif) ligand 9|C-C motif chemokine 9|MIP-1 gamma|macrophage inflammatory protein 1-gamma|macrophage inflammatory protein-related protein 2|small inducible cytokine A10|small inducible cytokine A9
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Ccl9, check out the Ccl9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ccl9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CCL9/Mip 1 Gamma PicoKine® Quick ELISA Kit (FEK1225)
Hello CJ!
No publications found for FEK1225
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Mouse CCL9/Mip 1 Gamma PicoKine® Quick ELISA Kit?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Mouse CCL9/Mip 1 Gamma PicoKine® Quick ELISA Kit
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
