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Product Info Summary
| SKU: | EK1225 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse CCL9/MIP-1 gamma ELISA Kit PicoKine®
SKU/Catalog Number
EK1225
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CCL9/MIP-1 gamma ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CCL9/MIP-1 gamma ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1225)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Q22-Q122
Sensitivity
<1 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
See datasheet of EK1225 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1225 is reactive to Ccl9 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1225 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Ccl9
Chemokine (C-C motif) ligand 9 (CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium (FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1 (the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1225-CAP | Anti-Mouse Ccl9 Pre-coated 96-well strip microplate | 1 |
| EK1225-ST | Mouse Ccl9 Standard | 2 vials, 10 ng/tube |
| EK1225-DA | Mouse Ccl9 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.030 | 0.111 | 0.197 | 0.369 | 0.680 | 1.252 | 1.783 | 2.150 |
Data Example Images
Click image to see more details
Mouse CCL9 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Ccl9, Dilution ratio of 1:1000, concentration in serum and plasma is 50-200 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CCL9/MIP-1 gamma ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 12 | 77 | 272 | 12 | 74 | 290 |
| Standard deviation | 0.86 | 4.69 | 19.04 | 0.9 | 4.88 | 22.33 |
| CV (%) | 7.2% | 6.1% | 7% | 7.5% | 6.6% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Ccl9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 12 | 12 | 12 | 11 | 11 | 0.43 | 3.9% |
| Sample 2 | 77 | 67 | 78 | 82 | 76 | 5.52 | 7.2% |
| Sample 3 | 272 | 286 | 279 | 302 | 284 | 11.12 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For Ccl9 (Source: Uniprot.Org, NCBI)
Gene Name
Ccl9
Full Name
C-C motif chemokine 9
Weight
13871 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 9;CCF18;Macrophage inflammatory protein 1-gamma;MIP-1-gamma;Macrophage inflammatory protein-related protein 2;MRP-2;Small-inducible cytokine A9;CCL9 (29-101);CCL9 (30-101);CCL9 (31-101);Ccl9;Mrp2, Scya10, Scya9; Ccl9|CCF18, MRP, MRP-2, Scy, Scya, Scya10, Scya9|chemokine (C-C motif) ligand 9|C-C motif chemokine 9|MIP-1 gamma|macrophage inflammatory protein 1-gamma|macrophage inflammatory protein-related protein 2|small inducible cytokine A10|small inducible cytokine A9
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Ccl9, check out the Ccl9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ccl9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CCL9/MIP-1 gamma ELISA Kit PicoKine® (EK1225)
Hello CJ!
EK1225 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Diet-induced obesity accelerates oral carcinogenesis by recruitment and functional enhancement of myeloid-derived suppressor cells
Metformin enhances gefitinib efficacy by interfering with interactions between tumor-associated macrophages and head and neck squamous cell carcinoma cells
Cloning and expression of nlpA gene as DNA vaccine candidate against Acinetobacter baumannii
Wu L, Du L, Ju Q, Chen Z, Ma Y, Bai T, Ji G, Wu Y, Liu Z, Shao Y, Peng X. Silencing TLR4/MyD88/NF-κB Signaling Pathway Alleviated Inflammation of Corneal Epithelial Cells Infected by ISE. Inflammation. 2020 Nov 10. doi: 10.1007/s10753-020-01363-1. Epub ah
Species: Mouse
EK1225 usage in article: APP:ELISA, SAMPLE:CORNEAL EPITHELIAL CELLS, DILUTION:NA
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Customer Q&As
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8 Customer Q&As for Mouse CCL9/MIP-1 gamma ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Ccl9 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Ccl9 antigen?
Verified Customer
Verified customer
Asked: 2020-03-29
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-03-29
Question
Q: Can Ccl9 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Dhawan
Verified customer
Asked: 2020-01-23
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-01-23
Question
Q: What is the optimal O.D. value for Ccl9 ELISA kit? I used your Ccl9 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Ccl9 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-02-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Ccl9 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of Ccl9 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2019-02-15
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
W. Bhatt
Verified customer
Asked: 2018-12-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-12-22
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Ccl9 using the EK1225 Mouse Ccl9 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-09-21
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-09-21
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-07-31
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-07-31
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-05-02
Question
Q: how to thaw whole blood sample for Ccl9 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-03-05
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Ccl9 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-03-05
