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Product Info Summary
| SKU: | EK2128 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Mouse Cathepsin E ELISA Kit PicoKine®
View all Cathepsin E ELISA kits
SKU/Catalog Number
EK2128
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Cathepsin E ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ctse in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Cathepsin E ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2128)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S60-P397
Sensitivity
<20 pg/ml
Assay Range
0.156 ng/ml - 10 ng/ml
Standard Dilution Instructions
See datasheet of EK2128 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2128 is reactive to Ctse in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2128 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cathepsin E
Cathepsin E is a protein that in humans is encoded by the CTSE gene. It is mapped to 1q32.1. The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. Cathepsin E is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen, and it also has an important role in immune responses.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2128-CAP | Anti-Mouse Ctse Pre-coated 96-well strip microplate | 1 |
| EK2128-ST | Mouse Ctse Standard | 2 vials, 10 ng/tube |
| EK2128-DA | Mouse Ctse Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(ng/ml) | 0 | 0.156 | 0.312 | 0.625 | 1.25 | 2.5 | 5 | 10 |
| O.D. | 0.041 | 0.073 | 0.092 | 0.145 | 0.301 | 0.547 | 1.146 | 1.854 |
Data Example Images
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Mouse Cathepsin E PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Cathepsin E ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 178 | 785 | 2784 | 191 | 805 | 2845 |
| Standard deviation | 7.30 | 34.54 | 141.98 | 10.51 | 31.40 | 173.55 |
| CV(%) | 4.1% | 4.4% | 5.1% | 5.5% | 3.9% | 6.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cathepsin E in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 178 | 191 | 188 | 163 | 180 | 12.06 | 6.7% |
| Sample 2 | 785 | 696 | 814 | 793 | 772 | 37.83 | 4.9% |
| Sample 3 | 2784 | 2863 | 2924 | 2652 | 2806 | 154.32 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For Ctse (Source: Uniprot.Org, NCBI)
Gene Name
Ctse
Full Name
Cathepsin E
Weight
42.794kDa
Superfamily
peptidase A1 family
Alternative Names
Ctse; Cathepsin E; EC 3.4.23.34 CTSE CATE cathepsin E cathepsin E|erythrocyte membrane aspartic proteinase|slow-moving proteinase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Ctse, check out the Ctse Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ctse: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Cathepsin E ELISA Kit PicoKine® (EK2128)
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