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- Table of Contents
Facts about Cathepsin E.
May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. .
Human | |
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Gene Name: | CTSE |
Uniprot: | P14091 |
Entrez: | 1510 |
Belongs to: |
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peptidase A1 family |
CATE; Cathepsin E; CTSE; EC 3.4.23; EC 3.4.23.34; erythrocyte membrane aspartic proteinase; slow-moving proteinase
Mass (kDA):
42.794 kDA
Human | |
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Location: | 1q32.1 |
Sequence: | 1; NC_000001.11 (206009264..206023899, complement) |
Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B- lymphocytes.
Endosome. The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome.
If you are interested in purchasing the anti-CD68 (Macrophage Marker) monoclonal antibody, you've come to the right place. In this article, you will learn about CTSE Marker Application in Immunohistochemistry and Western Blotting, and you'll also find out how the antibody performs on ELISA. After you have learned the basic application of the CTSE Marker, it's time to learn about the best uses of this product.
Boster Bio's Anti-CD68 (Macrophage Markers) Monoclonal Antibody recognizes a glycoprotein of 110 kDa that is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, and is also compatible with myeloid precursor cells and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are presumed to be of monocyte/macrophage origin. It shows a strong granular cytoplasmic staining in chronic and acute myeloid leukemias, and in some cases in true histiocytic neoplasia.
CD68 is a widely used macrophage marker. It is available in various sizes and conjugated with various Alexa Fluor dyes. It is recommended that you permeabilize the membrane before using this antibody. The recommended permeabilization buffer for this antibody is LeucopermTM (BUF09), a proprietary reagent. If you are preparing to use this monoclonal antibody in IHC applications, you should first centrifuge the solution in a buffer pH 7.5.
This monoclonal antibody is designed to identify macrophages that have infiltrated the heart. In CAD patients, CD68+ cells are present in adipose tissue with a higher density than in non-CAD patients. Besides CD68, the protein also plays a role in inflammatory responses. This is because FFA is secreted by adipocytes, which contributes to adipocyte lipolysis. Furthermore, macrophages aggregate in epicardial adipose tissue. Moreover, IL-6 and TNF-a increase in CAD patients.
This antibody targets both IL-17RA and IL-17RC, two proteins that recruit macrophages. They are important regulators of the immune system and contribute to the tumor microenvironment. In addition, IL-17 has an anti-tumor activity. When compared to IL-17-receptor monoclonal antibodies, Boster Bio's anti-CD68 (Macrophage Marker) antibody provides robust anti-inflammatory response.
Human CD68 is a 110-kD transmembrane glycoprotein. It is a member of the LAMP family, which includes LAMP-1 and LAMP-2, DC-LAMP, and brain and dendritic cell-associated (BAD)-LAMP. CD68 has a single LAMP domain and a Mucin-like domain. Its human ortholog contains 326 amino acids and shares eighty percent of its sequence with murine CD68.
The CTSE Marker has recently become a reliable adjunctive differentiation marker in immunohistochemistry. Its high reactivity is confirmed by both internal and external controls. Its applications in immunohistochemistry are rapidly growing in the diagnostic pathology field. Several authors, including Taylor CR, have proposed the development of an antibody laboratory manual for immunohistochemistry. Moreover, Swanson PE has written a practical primer for diagnostic immunohistochemistry. His work is based on studies involving 306.
This new mAb is purified from ascites fluid through a Protein G column and displays high specificity in ELISA, immunohistochemistry and western blot. The results are similar to those obtained with the D2-40 mAb. However, there is still considerable uncertainty regarding the efficacy of 5B3 for immunohistochemistry. Hence, the development of 5B3 mAb is warranted.
Immunohistochemistry is a valuable tool in pathology research. It is a valuable diagnostic tool, allowing researchers to identify tissue antigens and visualize the underlying biological processes. They can also help identify tissue-biomaterial interactions, including immune responses. Using specific antigens, antibodies can identify major cell sources and analyze cell phenotypic changes. The accuracy of immunohistochemistry is enhanced by validation and reproducibility.
CTSE (Cysteine Sulfate -Tyrosine Ester) Marker is a protein that is highly expressed in tumor tissues. The presence of this protein marker helps pathologists diagnose tumors. A comprehensive IHC staining regimen will increase the reproducibility of your results. However, it should be noted that CTSE is less quantitative than other immunohistochemistry assays.
CTSE has been reported to show high expression in colon cancers, and has recently been used to detect tumors in gastric mucosa. These studies suggest that CTSE is associated with aberrant gastric differentiation, and may be associated with colonic tumors. A recent study examined the immunoexpression of CTSE and other gastric-type markers in colorectal polyps. The findings suggest that CTSE is a reliable marker of inflammatory gastric cancer.
While traditional chromogenic mIHC relies on antibodies raised from different species, CTSE isotypes, and tissue types, with specific secondary antibodies for each. This approach has the advantage of reducing the number of tissue sections needed and enabling better understanding of the relationship between markers. Furthermore, CTSE is an excellent counterstain for the localization of primary antibodies. Its use in immunohistochemistry enables a faster turnaround time than traditional mIHC.
The CTSE marker is a candidate for gastric cancer. The expression of CTSE is correlated with CTX-M and MUC5AC in tumor tissues. Although CTSE expression has been shown to be correlated with these two markers, its significance in gastric cancer remains unknown. This article describes the validation of the CTSE marker on Western Blotting. Read on to learn how it can be used to further improve gastric cancer detection.
This technique uses antibodies to detect single proteins within protein mixtures. The protein mixture is then applied to a gel electrophoresis platform, separated according to size, charge, or other differences. The separated bands are then transferred to a carrier membrane, such as nitrocellulose, nylon, or PVDF. Antibodies can then bind to the proteins on immunoblots. This procedure is known as western blotting.
Validation of the CTSE Marker on an ELISA has been performed to validate the test for the detection of typhoid fever. To validate a test, it must have optimal and precise performance conditions. This is essential for any newly developed or modified diagnostic test, as it must be accurate, reproducible, and standardized to be of value for clinical practice. The following sections discuss the key characteristics of a validated ELISA.
First, choose a suitable ELISA protocol. The type of ELISA will be based on the sample type, the reagents available and the concentration of the analyte. The procedure should be as straightforward and uncomplicated as possible, since any error can cause a false result. There are two types of ELISA protocols: indirect and sandwith ELISA. Direct ELISA is a simple test based on antigen-based detection, while sandwith ELISA uses the samples as the targets.
The CTSE Marker has been validated on ELISAs for many applications. It is useful for detecting the presence of a single antigen at low concentrations. However, when multiple concentrations are performed, the sensitivity of the ELISA depends on the substrate used. If the substrate has a low concentration, a high-quality ELISA should give positive results. Similarly, if a low concentration is used to detect a high-concentration antigen, it will be interpreted as negative.
A sandwich ELISA is a good choice for low-concentration immunogenic substances, like typhoid fever. The sandwich ELISA contains a low concentration of the antigen, and is therefore the most sensitive method for measuring typhoid fever. It also enables researchers to test different concentrations of the same antigen without the use of complex methods. It uses surface-bound antibodies to detect typhoid fever.
The first step in an ELISA is coating the wells with antigen. The antigen is incubated until it adsorbs to the surface of the microtiter plate. It is important to understand how this adsorption process occurs as it depends on the hydrophobic interaction of the amino acid side chains with the surface of the plastic. The time of adsorption will vary depending on the temperature, pH of the coating buffer, and concentration of the antigen.
PMID: 2674141 by Azuma T., et al. Human gastric cathepsin E. Predicted sequence, localization to chromosome 1, and sequence homology with other aspartic proteinases.
PMID: 1370478 by Azuma T., et al. Human gastric cathepsin E gene. Multiple transcripts result from alternative polyadenylation of the primary transcripts of a single gene locus at 1q31-q32.