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Product Info Summary
| SKU: | EK1193 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Betacellulin/BTC ELISA Kit PicoKine®
View all Betacellulin/BTC ELISA kits
SKU/Catalog Number
EK1193
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Betacellulin/BTC ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Btc in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Betacellulin/BTC ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1193)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: D32-Y111
Sensitivity
<2 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
See datasheet of EK1193 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1193 is reactive to Btc in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1193 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Betacellulin/BTC
Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1193-CAP | Anti-Mouse Btc Pre-coated 96-well strip microplate | 1 |
| EK1193-ST | Mouse Btc Standard | 2 vials, 10 ng/tube |
| EK1193-DA | Mouse Btc Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.100 | 0.118 | 0.128 | 0.171 | 0.253 | 0.421 | 0.837 | 1.801 |
Data Example Images
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Mouse Betacellulin/BTC PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Betacellulin/BTC, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 7.8 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Betacellulin/BTC ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 11 | 59 | 318 | 10 | 63 | 326 |
| Standard deviation | 0.79 | 4.66 | 22.26 | 0.76 | 5.04 | 28.68 |
| CV (%) | 7.2% | 7.9% | 7% | 7.6% | 8% | 8.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Betacellulin/BTC in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 11 | 13 | 11 | 13 | 12 | 1 | 8.3% |
| Sample 2 | 59 | 66 | 65 | 58 | 62 | 3.53 | 5.6% |
| Sample 3 | 318 | 286 | 280 | 281 | 291 | 15.61 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For Btc (Source: Uniprot.Org, NCBI)
Gene Name
Btc
Full Name
Probetacellulin
Weight
19664 MW
Alternative Names
Probetacellulin;Betacellulin;BTC;Btc;Bcn; BTC betacellulin probetacellulin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Btc, check out the Btc Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Btc: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Betacellulin/BTC ELISA Kit PicoKine® (EK1193)
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Customer Q&As
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8 Customer Q&As for Mouse Betacellulin/BTC ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-11-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-11-23
Question
Q: What is the optimal O.D. value for Betacellulin ELISA kit? I used your Betacellulin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Betacellulin even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-07-10
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Betacellulin ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Betacellulin that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-07-10
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Betacellulin using the EK1193 Mouse Betacellulin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-06-11
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-06-11
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
N. Thomas
Verified customer
Asked: 2020-03-12
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-03-12
Question
Q: if the enzyme conjugated Betacellulin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Betacellulin antigen?
P. Adams
Verified customer
Asked: 2019-08-18
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-08-18
Question
Q: is it okay to use citrate plasma as samples in Mouse Betacellulin Picokine® ELISA Kit (Catalog # EK1193)?
Verified Customer
Verified customer
Asked: 2018-10-21
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Betacellulin causing disruption of its protein structure. Betacellulin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Betacellulin ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-10-21
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Betacellulin in Mouse cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
L. Anand
Verified customer
Asked: 2018-09-21
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Betacellulin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-09-21
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Betacellulin level in cell lysates.
Verified Customer
Verified customer
Asked: 2018-04-04
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-04-04
