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- Table of Contents
38 Citations 8 Q&As
34 Citations 7 Q&As
11 Citations 9 Q&As
5 Citations 17 Q&As
3 Citations 16 Q&As
4 Citations 16 Q&As
2 Citations 15 Q&As
4 Citations 2 Q&As
8 Citations
1 Citations
Facts about Adiponectin.
Antagonizes TNF-alpha by negatively regulating its expression in a variety of tissues such as liver and macrophages, and by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway.
Human | |
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Gene Name: | ADIPOQ |
Uniprot: | Q15848 |
Entrez: | 9370 |
Belongs to: |
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No superfamily |
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein
Mass (kDA):
26.414 kDA
Human | |
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Location: | 3q27.3 |
Sequence: | 3; NC_000003.12 (186842710..186858463) |
Synthesized exclusively by adipocytes and secreted into plasma.
Secreted.
If you're looking for an Anti-Adiponectin/ADIPOQ Marker, you've come to the right place. Here, you'll find out about Boster Bio and the ADIPOQ Marker. Additionally, you'll be taught about ELISA kits and validation. Finally, you'll be taught about Boster Bio's service to customers and customized services. What is Boster Bio, and how can I use it?
Boster Bio's ADIPOQ Marker has proved reliable in numerous applications. The ADIPOQ monoclonal antibody reacts with pig, mouse, and human antigens. Boster's antibodies are very specific to human, mouse and rat proteins. These antibodies can be used for immunohistochemistry as well as Western Blotting.
Boster Bio produces picogram-level ELISA kits and IHC-optimized polyclonal antibodies for a variety of research purposes. Their broad product line includes more than 12,000 antibodies validated for IHC, WB, and flow. All Boster antibodies are tested in a quantitative manner against 250 tissue samples as well as untransfected cell lines to ensure high affinity. The antibodies are also tested against known concentrations of recombinant Protein and human cell lines.
There are four types of ADIPOQ SNPs. The one that has 26.9 percent frequency is GGGGCCTT. However, the frequency of GGGGCCTT is very low in humans and is not significant in genetic models. In the end, ADIPOQ SNPs are the most useful in determining the presence of metabolic diseases and obesity. The ADIPOQ Marker is not only is sensitive and specific but also extremely precise and versatile.
Anti-Adiponectin/ADIPQ is a novel, soluble defense collagen protein that plays a critical role in regulating energy homeostasis and lipid and glucose metabolism. It is anti-inflammatory and is able to bind to a recognition motif on apoptotic cells. It aids in the elimination of apoptotic body by macrophages via a receptor-dependent pathway that is triggered by calreticulin.
Adiponectin interacts with calreticulin on the macrophages on their surface. Anti-calreticulin antibody binding to THP-1 macrophages has been inhibited by adiponectin. Furthermore, preincubation with adiponectin hinders its binding to the calreticulin. To determine whether anti-calreticulin antibody can bind to Adiponectin, THP-1 macrophages were pre-incubated with 200 mg/ml adiponectin. The anti-calreticulin antibody was then added to THP-1 macrophages, and flow cytometric analysis showed that the adiponectin-treated macrophages bound less calreticulin-specific antibody.
In addition to its role in the regulation of metabolism, adiponectin also involved in the reverse of insulin resistance in patients with obesity. Adiponectin gene overexpression can lead to increased the accumulation of lipids as well as glucose uptake and an increase in the metabolism of fatty acids. In the human body, adiponectin is found in fat cells as well in bone and muscle.
In research, recombinant adiponectin was equal to or more efficient than C1q, an enzyme that stimulates the uptake of apoptotic cells macrophages. Adiponectin stimulation of apoptotic cells ' uptake was dose-dependent, and was found to be saturated at 4 mg/ml. It also stimulated THP-1 macrophages to take up early apoptotic neutrophils. It did not affect the release of microbeads that were opsonized with IgG or BSA.
The Boster Bio Anti-Adiponectin/ADIPQ Marker has been validated in multiple platforms and tested on Human, Mouse, and Rat. Boster Bio has also validated the antibodies against known positive or negative samples on a variety platforms. The microplate format used for the antibody reagent can be found in a convenient 96 well strip microplate format. You can purchase blocking peptide for the Boster Bio Anti-Adiponectin/ADIPOQ Marker.
The Boster Bio ADIPOQ ELISA Kit is an ELISA-based assay for the detection of Adiponectin. This kit contains an antibody that is monoclonal to ADIPOQ. It reacts with humans, Mouse, and Rats and has been tested in WB, IHC, and has been confirmed. The Boster Bio ADIPOQ ELISA Kit is suitable for experiments in animal models.
This ELISA kit contains all buffers and Reagents. ELISA is a plate-based method that involves the linking of antibodies with enzymes in order to detect and detect the presence of specific molecules. Different formats use different antibody-antigen interactions. Boster's Picokine (tm) ELISA kit contains high-affinity antigens that can identify native forms proteins. The Boster ELISA kits are tested against relevant superfamilies and similar immunogenic proteins.
The Boster Bio ADIPOQ ELISA kit can be used for studies involving Adiponectin. It is constructed on the sandwich ELISA method. It includes an anti-mouse monoclonal antibody for Adiponectin pre-coated onto 96-well plates. The plates are then filled with standards and test samples. The biotinylated detection monoclonal antibodies are then added. The unbound conjugates are washed out with PBS or TBS buffer to eliminate Avidin-Biotin-Peroxidase complex.
The Boster Bio ADIPOQ ELISA Kit contains various components. First, the kit includes the human adiponectin/Acrp30 standard. It also contains an human ADIPOQ anti-body cocktail. It also contains six ml of sample buffer, 15ml wash buffer and 12ml AR1104 color-developing Reagent. Additionally, it comes with an adhesive for the plate and a multichannel pipette. For large samples the use of a multichannel pipette is advised.
A study of large Brazilian populations has shown that the ADIPOQ Marker can be associated with obesity. The ADIPOQ-rs182052 variant was linked with an increased risk of becoming obese. However, no connection was observed with other SNPs. The study's authors declare no conflict of interest. Further research is required to determine if the ADIPOQ marker plays any other role in determining overweight.
Each participant was given DNA samples. Genomic DNA was isolated using a commercial DNA extraction kit. The ADIPOQ gene was detected by a real-time polymerase chain reaction (PCR) assay. The PCR assay was conducted using an ABI Prism 7500 Real-Time PCR system. Positive and negative internal controls were included in the analysis. The resultant cDNA was purified from blood samples of healthy donors and patients with type II diabetes.
The hormone adiponectin is encoded by the ADIPOQ gene. Adiponectin levels are a strong genetic component. The ADIPOQ gene is composed of three exons and is located on chromosome3q27, which has been linked to susceptibility to metabolic syndrome as well as type 2 diabetes and cardiovascular disease. This marker is derived from this gene, and therefore, can be tested in patients.
The ADIPOQ gene is linked to the expression of adiponectin an adipocytokine that participates in energy homeostasis. Additionally, lower levels of Adiponectin stimulate the phosphorylation targeted proteins through AMPK which is a gene that plays a role in lipid and carbohydrate metabolism. The decrease in adiponectin levels can have many consequences on the body's metabolism, impacting many tissues and processes, including appetite and energy expenditure.
ADIPOQ is related to the function and expression of Adiponectin. This adipocytokine plays an important function in energy homeostasis. It influences the metabolism of a broad range of tissues . It also when it is decreased, it activates the protein AMPK which is essential in the process of metabolizing carbohydrate and lipids. These effects alter the metabolism of various tissues, extending energy-sucking processes and stimulating food intake. Studies have demonstrated that ADIPOQ mutations can result in an alteration in energy homeostasis.
Many studies have examined ADIPOQ variant rs2241766 in a particular population. The prevalence of this variant is less than ADIPOQ rs2241766 in other populations. However, these findings might not be applicable to all people. The ADIPOQ gene is found in a variety of tissues, among them the gastrointestinal tract which is a place of inflammation and abrasion.
PMID: 8619847 by Maeda K., et al. cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1).
PMID: 10095105 by Saito K., et al. Organization of the gene for gelatin-binding protein (GBP28).
*Showing only the more recent 20. More publications can be found for each product on its corresponding product page