LBP Lipopolysaccaride Human Recombinant Protein

LBP protein, Human

The Lipopolysaccharide Binding Protein is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The recombinant Human LBP was purified by his-tag with metal affinity purification with Talon and controlled by SDS page. Showing a 58kDa band on SDS-PAGE. Attention: His-tag has no protease site and can not be split off.

Product Info Summary

SKU: PROTP18428
Size: 2ug, 5ug, 10ug
Origin Species: Human
Source: CHO cells

Product Name

LBP Lipopolysaccaride Human Recombinant Protein

View all LBP recombinant proteins

SKU/Catalog Number

PROTP18428

Size

2ug, 5ug, 10ug

Description

The Lipopolysaccharide Binding Protein is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The recombinant Human LBP was purified by his-tag with metal affinity purification with Talon and controlled by SDS page. Showing a 58kDa band on SDS-PAGE. Attention: His-tag has no protease site and can not be split off.

Storage & Handling

Lyophilized LBP Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Cite This Product

LBP Lipopolysaccaride Human Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP18428)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Recombinant Human LBP was lyophilized from a protein solution (0.3mg/ml) containing phosphate-buffered saline, pH 7.2.

Predicted MW

53.384kDa

Reconstitution

Every 10µg of recombinant human LBP should be reconstituted using 33µl of sterile H2O. The solution can be than diluted with phosphate-buffered saline or other buffers.

Biological Activity

Up to 0.2 µg/ ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (FACS).

Reconstitution

Every 10µg of recombinant human LBP should be reconstituted using 33µl of sterile H2O. The solution can be than diluted with phosphate-buffered saline or other buffers.

Validation Images & Assay Conditions

Gene/Protein Information For LBP (Source: Uniprot.org, NCBI)

Gene Name

LBP

Full Name

Lipopolysaccharide-binding protein

Weight

53.384kDa

Superfamily

BPI/LBP/Plunc superfamily

Alternative Names

Lipopolysaccharide-binding protein; LBP; MGC22233 LBP BPIFD2 lipopolysaccharide binding protein lipopolysaccharide-binding protein|BPI fold containing family D, member 2|LPS-binding protein

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on LBP, check out the LBP Infographic

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Size

Total: $375

SKU:PROTP18428

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