Product Info Summary
SKU: | LS003682 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) (ITGAE) |
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Product info
Product Name
Integrin alpha E (ITGAE) (NM_002208) Human Over-expression Lysate
SKU/Catalog Number
LS003682
Size
100 µg
Description
Transient overexpression lysate of integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) (ITGAE)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
Integrin alpha E (ITGAE) (NM_002208) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003682)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
Integrin alpha E (ITGAE) Human Over-expression Lysates NM_002208.
Western blot validation of overexpression lysate (LS003682) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ITGAE (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ITGAE (Source: Uniprot.org, NCBI)
Gene Name
ITGAE
Full Name
Integrin alpha-E
Weight
130.159kDa
Superfamily
integrin alpha chain family
Alternative Names
antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103 antigen; CD103; HML-1 antigen; HUMINAE; Integrin alpha E; integrin alpha-E; Integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide); ITGAE; MGC141996; Mucosal lymphocyte 1 antigen ITGAE CD103, HUMINAE integrin subunit alpha E integrin alpha-E|HML-1 | CD103, human mucosal lymphocyte 1; alpha polypeptide|human mucosal lymphocyte 1, alpha polypeptide|integrin alpha-IEL|integrin, alpha E ( CD103, human mucosal lymphocyte 1; alpha polypeptide)|mucosal lymphocyte 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ITGAE, check out the ITGAE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ITGAE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Integrin alpha E (ITGAE) (NM_002208) Human Over-expression Lysate (LS003682)
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