VCAM1 Antibodies

Vascular cell adhesion protein 1 (VCAM1)

Significant in cell-cell recognition. Appears to work in leukocyte-endothelial cell adhesion.

Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Gene Information

Human
Gene Name:	VCAM1
Uniprot:	P19320
Entrez:	7412

Family

Belongs to:
No superfamily

Alternative Names

CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

Molecular Weight

Mass (kDA):
81.276 kDA

Genomic Context

Human
Location:	1p21.2
Sequence:	1; NC_000001.11 (100719640..100739045)

Tissue Specificity

Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.

Subcellular Localizations

Membrane; Single-pass type I membrane protein.

Diseases

• Tissue Adhesions
• Inflammation
• Atherosclerosis
• Neoplasms
• Malignant Neoplasms
• Cardiovascular Diseases
• Neoplasm Metastasis
• Carcinoma
• Cerebrovascular Accident
• Diabetes Mellitus
• Tumor Angiogenesis
• Inflammatory Response

• Infarction
• Myocardial Infarction
• Ischemia
• Pathologic Neovascularization
• Hypertensive Disease
• Obesity
• Leukemia

Pathways

• Cell Adhesion
• Pathogenesis
• Immune Response
• Inflammatory Response
• Angiogenesis
• Cell Activation
• Cell Proliferation
• Secretion
• Endothelial Cell Activation
• Cell Cycle
• Vasculogenesis
• Cell Division
• Transport

• Reverse Transcription
• Senescence
• Cell Growth
• Aging
• Tube Formation
• Neurogenesis
• Hypersensitivity

PTMs

• Phosphorylation
• Reduction

• Cleavage
• Glycosylation

• Deglycosylation

Research Areas

• Cancer
• Mesenchymal Stem Cell Markers
• Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/VCAM1

Best Uses of the VCAM1 Marker in Biological Assays

Biological assays are the most effective way to determine the VCAM1 marker's properties. Boster Bio offers this marker to help scientists find the ideal conditions for a specific species or application. Researchers from all over the world can submit their findings to earn product credits. These benefits aren't only for scientists in the US and UK however, they are also available to scientists across the globe.

Anti-VCAM1 Marker

The Anti-VCAM1 Marker in Bostr Bio has a remarkably good performance in immunohistochemistry. The molecule is a physiologic ligand. It interacts with immature, double-positive cells within the cortex. The results demonstrate a strong correlation between this molecule and the IL-4. However, there is a lot of confusion about the mechanism of the marker.

Expression of VCAM-1 in TECs was first examined using human thymic epithelial cell lines. VCAM-1 is expressed in human thymic epithelial cell (TECs) and is upregulated by IL-4 when stimulated by IL-4. Monolayers of freshly prepared thymocytes may be able to bind TECs with antibodies against anti-a4b1 or anti-VCAM-1.

Double LPS stimulation increased the expression of VCAM-1 in various organs including the liver and kidney. VCAM-1 expression was significantly higher in all vascular segments following LPS challenge. However the non-targeted IgG was recruited into glomeruli, which could be the reason for the increased leakage. Boster Bio's anti-VCAM1 marker was extremely efficient in identifying these vessel cells and their function within the body.

The Boster Bio Anti-VCAM1 Marker found in Bostr Bio has been tested for its capacity to detect VCAM1 protein in cell culture and other tissue samples. It is a monoclonal antibody that reacts with VCAM1 protein in many animal specimens. The Boster Bio Anti-VCAM1 Marker from Boster Bio makes use of rabbit and mouse cells as the model systems to develop VCAM1 antibodies. A complex biochemical process is required to aid in the production of antibodies. This involves the use the chemical ligand.

A series of serial images compiled using confocal z-plane analysis proved that the VCAM-1 protein staining was true colocalized. This was achieved by superimposing images of two different fluorescence detectors. The results from Series I are representative of the CMJ Series II are for the cortex. The anti-VCAM1 marker in Boster Bio is effective for the detection of VCAM-1 in CMJ, but not in the medulla.

This is the very first demonstration of the expression of VCAM-1 protein on epithelial cell epithelial cells. It can be used to study the interaction between mature and immature thymocytes. The limited expression of FN suggests that it has functional significance for the thymocyte development and migration. The VCAM1 FN protein is a significant marker for T cell selection and migration in the thymus.

Bioassays

The use of Boster Bio VCAM1 biological assay kits is a great method of determining the amount of VCAM1 in human blood. This test can be used in both ELISA and immunohistochemistry applications. Boster Bio VCAM1 is the most reliable, accurate and user-friendly biological assay kit. It also provides customized services, including BeNeLux delivery.

The study revealed that VCAM-1 knockdown decreased TGF-b1-mediated invasion, migration, and proliferation of human endometriotic cell lines. These findings suggest that VCAM-1 may be a potential therapeutic target in endometriosis. However this study isn't conclusive. The next step to understand the role of VCAM1 in endometriosis is testing the effects of various drugs on the protein.

Biological assays utilize antibodies to determine VCAM1 presence in blood. Boster Bio uses rabbit and mouse as model species in the creation of its VCAM1 antibodies. This protein interacts to integrin beta-4 beta-1 (ITGA4/ITGB1) and could be involved with immune responses or leukocyte movement to sites of inflammation. Researchers can utilize blood VCAM1 to determine it.

The MBS175995 ELISA kit detects VCAM-1 by detecting native VCAM1 antibodies. This kit is ideal for analysing undiluted body fluids, cell culture supernatants, as well as tissues. It is an excellent option for research studies because of its high sensitivity and lack of cross-reactivity. It is also useful in biotechnological development. For example, the Boster VCAM1 ELISA Kit (MBS175995) can detect VCAM-1 in human blood samples.

Research: Application

What is the definition of application in Research? What is applied research is research that applies scientific knowledge to real-world scenarios. It is a method of applying knowledge gained from scientific research to solve specific problems or improving processes in organizations. It can be done in areas such as material science, chemistry, or physics science. For instance, it can be used in microbiology or thermodynamics, or to treat specific diseases. Research in applied areas is also conducted by industrial psychologists. These kinds of researchers combine psychology with other theories to help improve workplace performance.

Research is an essential component of pharmaceutical companies' capability to come up with new treatments. They study diseases, synthesize new molecules, then test them to see which one is most efficient. The process can take years or even decades. There are many kinds of research, from professional and scientific. Every type of research is designed to improve knowledge of the subject or industry. Let's take a look at some of the most well-known kinds to be aware of their applications. What is Application in Research?

Basic research, however, focuses on expanding knowledge through the study of various concepts. Contrary to that, applied research is designed to solve specific problems or discover cures for illnesses. It's also specific to a particular problem and focuses on knowledge expansion. It applies the principles of basic research to improve the quality of a particular situation or issue. Applied research is a method to provide evidence that can be utilized to solve practical problems. In reality the application of applied research is more practical as it focuses on specific problems rather than generalization.

When studying an subject or area, ICT can help researchers to discover issues and devise innovative solutions. Applied research can also be described as scientific method of inquiry or contractual research. It is the process of applying scientific principles to real-world issues. Researchers begin by identifying the issue and then formulate a research hypothesis. Then , they test their theories by using experiments or simulations. The aim of conducting research is to improve research quality and to find practical solutions.

Refer to

Neurons express the VCAM1 marker. This marker contributes to myelination, regulates CD69 and is involved in the beginning of the process. It is primarily expressed in the CNS and regulates the quantity of CD69. It also plays a role in neuronal differentiation. There are numerous possible functions of VCAM1.

VCAM1 is expressed in a variety of tissues and is active in the tissues. In contrast the expression of VCAM1 is decreased in the peripheral region. This is consistent with the absence of VCAM-1 expression in peritoneal fluid of patients with thioglycolate-induced aseptic peritonitis. Therefore VCAM-1 is an essential biomarker in the diagnosis of this condition.

The VCAM1 marker is a crucial marker in the study of vascular permeability. It also acts as an adhesion molecule in cells. It is located on the surface of vascular endothelium. It is able to interact with the Integrin a4b1 in the vascular cells. Additionally, it binds to VLA-4 through the first and fourth Ig-like domains. VCAM1 could be a key component of lymphocyte transendothelial mobility. More research is needed.

The researchers found that age-related plasma boosts the amount of VCAM1 in the brain, which accelerates aging. Untreated mice showed normal brain aging due higher levels of VCAM1 in the group with a higher age. The aging process was less pronounced in mice treated with antibodies against VCAM1. The behavioral studies were also discussed in the Keystone talk given by Wyss Coray and bioRkhiv's paper. These findings are crucial for studying the mechanisms that drive the human ageing process.