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Product Info Summary
| SKU: | EK1169 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human S100A12/En Rage ELISA Kit PicoKine®
View all EN-RAGE/S100A12 ELISA kits
SKU/Catalog Number
EK1169
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human S100A12/En Rage ELISA Kit PicoKine® (96 Tests). Quantitate Human S100A12 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human S100A12/En Rage ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1169)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: M1-E92
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1169 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1169 is reactive to S100A12 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1169 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of EN-RAGE/S100A12
S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene. It is also known as calgranulin C. By direct R-banding FISH, the S100A12 gene is mapped to chromosome 1q21.2-q22. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. And this protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1169-CAP | Anti-Human S100A12 Pre-coated 96-well strip microplate | 1 |
| EK1169-ST | Human S100A12 Standard | 2 vials, 10 ng/tube |
| EK1169-DA | Human S100A12 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.004 | 0.096 | 0.171 | 0.321 | 0.759 | 1.238 | 1.701 | 2.057 |
Data Example Images
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Human S100A12 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect EN-RAGE/S100A12, Dilution ratio of 1:300, concentration in serum and plasma is 1.3-85.2 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human S100A12/En Rage ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 58 | 265 | 947 | 56 | 247 | 855 |
| Standard deviation | 2.32 | 20.4 | 67.23 | 3.08 | 23.46 | 68.4 |
| CV (%) | 4% | 7.7% | 7.1% | 5.5% | 9.5% | 8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of EN-RAGE/S100A12 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 58 | 61 | 58 | 60 | 59 | 1.29 | 2.1% |
| Sample 2 | 265 | 274 | 252 | 278 | 267 | 9.98 | 3.7% |
| Sample 3 | 947 | 981 | 966 | 1077 | 992 | 50.11 | 5% |
Protein Target Info & Infographic
Gene/Protein Information For S100A12 (Source: Uniprot.Org, NCBI)
Gene Name
S100A12
Full Name
Protein S100-A12
Weight
10575 MW
Superfamily
S-100 family
Alternative Names
Protein S100-A12;CGRP;Calcium-binding protein in amniotic fluid 1;CAAF1;Calgranulin-C;CAGC;Extracellular newly identified RAGE-binding protein;EN-RAGE;Migration inhibitory factor-related protein 6;MRP-6;p6;Neutrophil S100 protein;S100 calcium-binding protein A12;Calcitermin;S100A12; S100A12 CAAF1, CAGC, CGRP, ENRAGE, MRP-6, MRP6, p6 S100 calcium binding protein A12 protein S100-A12|EN-RAGE|calcitermin|calcium-binding protein in amniotic fluid 1|calgranulin C|extracellular newly RAGE-binding protein|migration inhibitory factor-related protein 6|neutrophil S100 protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on S100A12, check out the S100A12 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for S100A12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human S100A12/En Rage ELISA Kit PicoKine® (EK1169)
Hello CJ!
EK1169 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of Transcutaneous Electrical Acupoint Stimulation on Postoperative Cognitive Decline in Elderly Patients: A Pilot Study
Liu T,Yin C,Li Y,Gao F,Yu L,Wang Z,Wang Q.Effects of Transcutaneous Electrical Acupoint Stimulation on Postoperative Cognitive Decline in Elderly Patients: A Pilot Study.Clin Interv Aging.2021 May 3;16:757-765.doi:10.2147/CIA.S309082.PMID:33976542;PMCID:PMC8106456.
Species: Human
EK1169 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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11 Customer Q&As for Human S100A12/En Rage ELISA Kit PicoKine®
Question
Does EK1169 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Human S100A12/En Rage ELISA Kit PicoKine® (EK1169) doesn't contain native human biological materials. doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: Can EN-RAGE ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-08-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-08-16
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-10-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-10-12
Question
Q: how do I analyze ELISA data? I have performed EN-RAGE level in cell lysates.
H. Huang
Verified customer
Asked: 2019-08-19
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-08-19
Question
Q: is it okay to use citrate plasma as samples in Human EN-RAGE Picokine® ELISA Kit (Catalog # EK1169)?
Z. Carter
Verified customer
Asked: 2019-06-15
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of EN-RAGE causing disruption of its protein structure. EN-RAGE may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the EN-RAGE ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-06-15
Question
Q: how can I thaw whole blood sample for EN-RAGE ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test EN-RAGE for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-03-11
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-08-20
Question
Q: What is the optimal O.D. value for EN-RAGE ELISA kit? I performed your EN-RAGE ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain EN-RAGE even though the O.D. values are not very high?
W. Miller
Verified customer
Asked: 2018-01-12
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this EN-RAGE ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of EN-RAGE that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-01-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Wang
Verified customer
Asked: 2017-10-18
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-10-18
Question
The customer wants to know the suggested dilution ratio for human plasma samples for EK1169.
Verified Customer
Verified customer
Asked: 2015-10-02
Answer
COA is sent.
Boster Scientific Support
Answered: 2015-10-02
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-07-01
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2015-07-01
