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Product Info Summary
| SKU: | A15336 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-RSBN1 Antibody (N-term)
SKU/Catalog Number
A15336
Size
80 µl
Description
Boster Bio Anti-RSBN1 Antibody (N-term) (Catalog # A15336). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RSBN1 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15336)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RSBN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-78 amino acids from the N-terminal region of human RSBN1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15336 is reactive to RSBN1 in Human
Reconstitution
Calculated molecular weight
90072 Da
Background of RSBN1
PSMAL has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity (By similarity). PSMAL exhibits a dipeptidyl-peptidase IV type activity
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15336 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-RSBN1 Antibody (N-term) at 1:2000 dilution
Lane 1: human liver lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: HL-60 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 90 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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RSBN1 Antibody (N-term) (Cat. #A15336) western blot analysis in Jurkat cell line lysates (35ug/lane). This demonstrates the RSBN1 antibody detected the RSBN1 protein (arrow).
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All lanes : Anti-RSBN1 Antibody (N-term) at 1:2000 dilution
Lane 1: CCRF-CEM whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: human liver lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: HL-60 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 90 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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RSBN1 Antibody (N-term) (Cat. #A15336)immunohistochemistry analysis in formalin fixed and paraffin embedded human plecenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RSBN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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A15336 staining RSBN1 in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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A15336 staining RSBN1 in human colon tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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RSBN1 Antibody (N-term) (Cat. #A15336) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For RSBN1 (Source: Uniprot.org, NCBI)
Gene Name
RSBN1
Full Name
Lysine-specific demethylase 9
Weight
90072 Da
Superfamily
round spermatid basic protein 1 family
Alternative Names
Round spermatid basic protein 1, RSBN1 RSBN1 KDM9, ROSBIN round spermatid basic protein 1 lysine-specific demethylase 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RSBN1, check out the RSBN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RSBN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RSBN1 Antibody (N-term) (A15336)
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