Product Info Summary
SKU: | PROTP10600-8 |
---|---|
Size: | 5ug,20ug,100ug |
Origin Species: | Human |
Source: | Escherichia coli |
Application: | Cell Culture |
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Product info
Product Name
Human recombinant TGF beta 3 (Transforming growth factor beta 3) protein, AF
View all TGF-beta 3 recombinant proteins
SKU/Catalog Number
PROTP10600-8
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Transforming Growth Factors-Beta 3 (TGF-beta 3) is belonging to the TGF-β superfamily TGF-β3 is 86%, 91% similar to TGF-β1 and TGF-β2. TGF-beta 3 is a 12.8 kDa protein containing 412 amino acids, which could inhibitor of DNA synthesis, increases cellular proliferation, essential mediator of EMT in cardiac morphogenesis, and up-regulated by milk stasis and induces apoptosis in mammary gland epithelium during involution.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant TGF beta 3 (Transforming growth factor beta 3) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP10600-8)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
47.328kDa
Molecular weight
The protein has a calculated MW of 13.66 kDa. The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED₅₀ for this effect is <50 pg/mL. The specific activity of recombinant human TGF beta 3 is > 2 x 10⁷ IU/mg.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile 10 mM HCl to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
Click image to see more details
SDS- PAGE analysis of recombinant human TGF beta 3
Protein Target Info & Infographic
Gene/Protein Information For TGFB3 (Source: Uniprot.org, NCBI)
Gene Name
TGFB3
Full Name
Transforming growth factor beta-3 proprotein
Weight
47.328kDa
Superfamily
TGF-beta family
Alternative Names
ARVD, ARVD1, LDS5, RNHF, TGFB3, TGF-B3 TGFB3 ARVD, ARVD1, LDS5, RNHF, TGF-beta3 transforming growth factor beta 3 transforming growth factor beta-3 proprotein|prepro-transforming growth factor beta-3
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TGFB3, check out the TGFB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TGFB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant TGF beta 3 (Transforming growth factor beta 3) protein, AF (PROTP10600-8)
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