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Product Info Summary
| SKU: | PROTP09603-8 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Human recombinant M-CSF (Macrophage colony stimulating factor) protein, AF
View all M-CSF recombinant proteins
SKU/Catalog Number
PROTP09603-8
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Macrophage Colony Stimulating Factor (M-CSF) is a 18.54 kDa member of hematopoietic Growth Factors with 159 amino acid residues. M-CSF produced by osteoblasts and osteoblast precursors. M-CSF stimulates the growth and differentiation of the monocyte lineage, and promotes the survival, proliferation, and functions of mature monocytes/macrophages.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant M-CSF (Macrophage colony stimulating factor) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP09603-8)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
60.179kDa
Molecular weight
The protein has a calculated MW of 13.34 kDa. The protein migrates as 13-26 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce proliferation in NFS-60 cells. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human M-CSF is approximately >2.5x 10⁸ IU/mg.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQGHERQSEGS with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human M-CSF
Protein Target Info & Infographic
Gene/Protein Information For CSF1 (Source: Uniprot.org, NCBI)
Gene Name
CSF1
Full Name
Macrophage colony-stimulating factor 1
Weight
60.179kDa
Alternative Names
CSF-1, MGI-IM CSF1 CSF-1, MCSF colony stimulating factor 1 macrophage colony-stimulating factor 1|colony stimulating factor 1 (macrophage)|lanimostim|macrophage colony stimulating factor 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CSF1, check out the CSF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant M-CSF (Macrophage colony stimulating factor) protein, AF (PROTP09603-8)
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