Human recombinant IL-9 (Interleukin-9) protein, AF

IL-9 protein, Human

Interleukin 9 (IL-9) is a pleiotropic cytokine that had pleiotropic functions in the immune system, has a molecular mass of 14.5 kDa. The major source of IL-9 is T lymphocytes. It is secreted by CD4+ helper cells that acts as a regulator of a variety of hematopoietic cells.

Product Info Summary

SKU: PROTP15248-6
Size: 5ug,20ug,100ug,500ug,1mg
Origin Species: Human
Source: Escherichia coli
Application: Cell Culture

Product Name

Human recombinant IL-9 (Interleukin-9) protein, AF

View all IL-9 recombinant proteins

SKU/Catalog Number

PROTP15248-6

Size

5ug,20ug,100ug,500ug,1mg

Tag

His Tag (N-term)

Description

Interleukin 9 (IL-9) is a pleiotropic cytokine that had pleiotropic functions in the immune system, has a molecular mass of 14.5 kDa. The major source of IL-9 is T lymphocytes. It is secreted by CD4+ helper cells that acts as a regulator of a variety of hematopoietic cells.

Storage & Handling

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

Cite This Product

Human recombinant IL-9 (Interleukin-9) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP15248-6)

Form

Lyophilized

Formulation

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.

Purity

>98% as determined by SDS-PAGE.

Predicted MW

15.909kDa

Molecular weight

The protein has a calculated MW of 14.93 kDa. The protein migrates as 13-17 kDa under reducing condition (SDS-PAGE analysis).

Activity

Measure by its ability to induce proliferation in MO7e cells. The ED₅₀ for this effect is <0.25 ng/mL. The specific activity of recombinant human IL-9 is approximately >5 x10⁶ IU/ mg.

Endotoxin

<0.01 EU per 1 μg of the protein by the LAL method.

Amino Acid Sequence

QGCPTLAGILDINFLINKMQEDPASKCHCSANVTSCLCLGIPSDNCTRPCFSERLSQMTNTTMQTRYPLIFSRVKKSVEVLKNNKCPYFSCEQPCNQTTAGNALTFLKSLLEIFQKEKMRGMRGKI with polyhistidine tag at the N-terminus.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Validation Images & Assay Conditions

Gene/Protein Information For IL9 (Source: Uniprot.org, NCBI)

Gene Name

IL9

Full Name

Interleukin-9

Weight

15.909kDa

Superfamily

IL-7/IL-9 family

Alternative Names

p40 cytokine, T-cell growth factor p40, HP40 IL9 HP40, IL-9, P40 interleukin 9 interleukin-9|T-cell growth factor p40|cytokine P40|homolog of mouse T cell and mast cell growth factor 40|p40 T-cell and mast cell growth factor|p40 cytokine

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on IL9, check out the IL9 Infographic

IL9 infographic

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In this infographic, you will see the following information for IL9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $77

SKU:PROTP15248-6

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