Anti- IL-9 Monoclonal Antibody

Product Name

Anti- IL-9 Monoclonal Antibody

View all IL-9 Antibodies

SKU/Catalog Number

M02925

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti- IL-9 Monoclonal Antibody catalog # M02925. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti- IL-9 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02925)

Host

Mouse

Contents

PBS, pH 7.0. Contains no stabilizers or preservatives

Clonality

Monoclonal

Clone Number

AFID-9

Isotype

Mouse IgG2b, κ

Immunogen

Human IL-9 coupled to ovalbumin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M02925 is reactive to IL9 in Mouse, Rat

Applications

M02925 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

16 kDa

Calculated molecular weight

46663 MW

Background of IL-9

The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5&mu:g/ml, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of IL-9 using anti-IL-9 antibody (M02925).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse thymus tissue lysates,
Lane 4: mouse RAW264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IL-9 antigen affinity purified monoclonal antibody (Catalog # M02925) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for IL-9 at approximately 16KD. The expected band size for IL-9 is at 16KD.

Gene/Protein Information For IL9 (Source: Uniprot.org, NCBI)

Alternative Names

Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40 IL9 HP40, IL-9, P40 interleukin 9 interleukin-9|T-cell growth factor p40|cytokine P40|homolog of mouse T cell and mast cell growth factor 40|p40 T-cell and mast cell growth factor|p40 cytokine

For more info on IL9, check out the IL9 Infographic

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In this infographic, you will see the following information for IL9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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