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Product Info Summary
| SKU: | PROTP61328-2 |
|---|---|
| Size: | 5ug,20ug,100ug,500ug,1mg |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Human recombinant FGF-12 (Fibroblast growth factor-12) protein, AF
View all FGF-12 recombinant proteins
SKU/Catalog Number
PROTP61328-2
Size
5ug,20ug,100ug,500ug,1mg
Tag
His Tag (C-term)
Description
Fibroblast Growth Factors-12 (FGF-12) is a 20.4 kDa member of the fibroblast Growth Factors with 181 amino acid residues. FGF-12 is mainly expressed from inhibitory neurons, oligodendrocyte precursor cells, excitatory neurons, cardiomyocytes. Involved in nervous system development, function, and positive regulation of voltage-gated sodium channel activity. FGF-12 function in the development of the central and peripheral nervous systems, connective tissue of the skeleton, and the myocardia of the heart.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant FGF-12 (Fibroblast growth factor-12) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP61328-2)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
27.399kDa
Molecular weight
The protein has a calculated MW of 21.23 kDa. The protein migrates as 21 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2 ng/mL.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MESKEPQLKGIVTRLFSQQGYFLQMHPDGTIDGTKDENSDYTLFNLIPVGLRVVAIQGVKASLYVAMNGEGYLYSSDVFTPECKFKESVFENYYVIYSSTLYRQQESGRAWFLGLNKEGQIMKGNRVKKTKPSSHFVPKPIEVCMYREPSLHEIGEKQGRSRKSSGTPTMNGGKVVNQDSTwith polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human FGF-12
Protein Target Info & Infographic
Gene/Protein Information For FGF12 (Source: Uniprot.org, NCBI)
Gene Name
FGF12
Full Name
Fibroblast growth factor 12
Weight
27.399kDa
Superfamily
heparin-binding growth factors family
Alternative Names
EIEE47B, FHF1 FGF12 DEE47, EIEE47B, FHF1, FGF12 fibroblast growth factor 12 fibroblast growth factor 12|fibroblast growth factor 12B|fibroblast growth factor FGF-12b|fibroblast growth factor homologous factor 1|myocyte-activating factor
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on FGF12, check out the FGF12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant FGF-12 (Fibroblast growth factor-12) protein, AF (PROTP61328-2)
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