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Product Info Summary
| SKU: | PROTP13500-5 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Human recombinant CCL2 (C-C motif chemokine ligand 2) protein, AF
View all CCL2 recombinant proteins
SKU/Catalog Number
PROTP13500-5
Size
5ug,20ug,100ug
Tag
His Tag (N-term)
Description
C-C Motif Chemokine Ligand 2 (CCL2) is an 8.44 kDa cytokine with 76 amino acid residues and is also known as monocyte chemoattractant protein 1 (MCP1). The CCL2 secreted from monocytes, macrophages, and dendritic cells. Upon binding with CCR4, it regulates many biological functions, such as recruitments of monocytes, memory T cells, and dendritic cells, response to TNF-α and Interferon-γ, angiogenesis, cellular homeostasis, and cytoskeleton organization.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant CCL2 (C-C motif chemokine ligand 2) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP13500-5)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>95% as determined by SDS-PAGE.
Predicted MW
11.025kDa
Molecular weight
The protein has a calculated MW of 9.49 kDa. The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to chemoattract BaF3 cells transfected with human CCR2A. The ED₅₀ for this effect is <20 ng/mL.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT with polyhistidine tag at the N-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human CCL2
Protein Target Info & Infographic
Gene/Protein Information For CCL2 (Source: Uniprot.org, NCBI)
Gene Name
CCL2
Full Name
C-C motif chemokine 2
Weight
11.025kDa
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
Monocyte Chemotactic Protein-1,MCP-1 CCL2 GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF C-C motif chemokine ligand 2 C-C motif chemokine 2|chemokine (C-C motif) ligand 2|monocyte chemoattractant protein-1|monocyte chemotactic and activating factor|monocyte chemotactic protein 1|monocyte secretory protein JE|small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)|small inducible cytokine subfamily A (Cys-Cys), member 2|small-inducible cytokine A2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CCL2, check out the CCL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant CCL2 (C-C motif chemokine ligand 2) protein, AF (PROTP13500-5)
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