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Product Info Summary
| SKU: | FEK1329 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human Chemerin/RARRES2 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1329
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Chemerin/RARRES2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human Chemerin/RARRES2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human Chemerin/RARRES2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1329)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: V17-S163
Sensitivity
<20 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1329 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1329 is reactive to RARRES2 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK1329 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Chemerin
Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1(also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1329-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1329-ST | Human Chemerin Standard | 2 | 50 ng/tube |
| FEK1329-A | Human RARRES2 Antibody A | 1 | 3ml |
| FEK1329-B | Human RARRES2 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 |
| O.D. | 0.013 | 0.119 | 0.195 | 0.356 | 0.623 | 1.012 | 1.389 | 1.802 |
Data Example Images
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Human RARRES2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Chemerin/RARRES2 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 118 | 754 | 3266 | 128 | 779 | 3354 |
| Standard deviation | 6.49 | 47.5 | 192.69 | 6.66 | 57.65 | 197.89 |
| CV(%) | 5.5% | 6.3% | 5.9% | 5.2% | 7.4% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Chemerin in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 118 | 122 | 103 | 115 | 115 | 6.76 | 5.9% |
| Sample 2 | 754 | 766 | 687 | 729 | 734 | 27.16 | 3.7% |
| Sample 3 | 3266 | 2789 | 2969 | 3381 | 3101 | 192.28 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For RARRES2 (Source: Uniprot.Org, NCBI)
Gene Name
RARRES2
Full Name
Retinoic acid receptor responder protein 2
Weight
18.618kDa
Alternative Names
Chemerin; RARRES2; RAR-responsive protein TIG2; retinoic acid receptor responder (tazarotene induced) 2; retinoic acid receptor responder protein 2; Tazarotene-induced gene 2 protein; TIG-2; TIG2HP10433 RARRES2 HP10433, TIG2 retinoic acid receptor responder 2 retinoic acid receptor responder protein 2|RAR-responsive protein TIG2|chemerin|retinoic acid receptor responder (tazarotene induced) 2|tazarotene-induced gene 2 protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on RARRES2, check out the RARRES2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RARRES2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Chemerin/RARRES2 PicoKine® Quick ELISA Kit (FEK1329)
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