Human CD206/CLEC13D PicoKine® Quick ELISA Kit

Product Name

Human CD206/CLEC13D PicoKine® Quick ELISA Kit

See all MMR/CD206/Mannose Receptor products

SKU/Catalog Number

FEK2049

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD206/CLEC13D PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD206/CLEC13D in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human CD206/CLEC13D PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK2049)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: L19-K1383

Sensitivity

<50 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Standard Dilution Instructions

See datasheet of FEK2049 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK2049 is reactive to MRC1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Application Guarantee

FEK2049 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of MMR/CD206/Mannose Receptor

The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages and immature dendritic cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205). This gene is mapped to 10p12.33. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.

Kit Components

Catalog number	Description	Quantity	Volume
FEK2049-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK2049-ST	Human MMR/CD206/Mannose Receptor Standard	2	50 ng/tube
FEK2049-A	Human MRC1 Antibody A	1	3ml
FEK2049-B	Human MRC1 Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.034	0.179	0.285	0.502	0.883	1.461	2.019	2.326

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD206/CLEC13D PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMR/CD206/Mannose Receptor in ELISA kits from four different production batches/lots.

Gene/Protein Information For MRC1 (Source: Uniprot.Org, NCBI)

Alternative Names

CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1 MRC1 CD206, CLEC13D, CLEC13DL, MMRL1, bA541I19.1, hMR, MRC1 mannose receptor C-type 1 macrophage mannose receptor 1|C-type lectin domain family 13 member D|human mannose receptor|macrophage mannose receptor 1-like protein 1|mannose receptor, C type 1-like 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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