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Product Info Summary
| SKU: | EK2020 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Matrilin-2 ELISA Kit PicoKine®
View all Matrilin-2 ELISA kits
SKU/Catalog Number
EK2020
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Matrilin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human MATN2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Matrilin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2020)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: R24-R956
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2020 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2020 is reactive to MATN2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2020 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Matrilin-2
Matrilin-2 is a protein that in humans is encoded by the MATN2 gene. This gene is mapped to 8q22.1-q22.2. This gene encodes a member of the von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains five von Willebrand factor A domains. The specific function of this gene has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2020-CAP | Anti-Human MATN2 Pre-coated 96-well strip microplate | 1 |
| EK2020-ST | Human MATN2 Standard | 2 vials, 10 ng/tube |
| EK2020-DA | Human MATN2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.044 | 0.132 | 0.212 | 0.346 | 0.591 | 1.063 | 1.659 | 2.189 |
Data Example Images
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Human Matrilin-2 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Matrilin-2, For serum, dilution ratio is 1:1 and concentration is around 1000 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is around 300 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Matrilin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 41 | 183 | 654 | 49 | 212 | 719 |
| Standard deviation | 1.97 | 10.43 | 30.08 | 2.50 | 12.51 | 46.02 |
| CV (%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Matrilin-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 41 | 34 | 37 | 41 | 38 | 2.94 | 7.7% |
| Sample 2 | 183 | 175 | 170 | 188 | 179 | 6.96 | 3.8% |
| Sample 3 | 654 | 569 | 621 | 671 | 628 | 38.89 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For MATN2 (Source: Uniprot.Org, NCBI)
Gene Name
MATN2
Full Name
Matrilin-2
Weight
106.837kDa
Alternative Names
Matrilin-2; MATN2; UNQ193; PRO219 MATN2 matrilin 2 matrilin-2|testis tissue sperm-binding protein Li 94mP
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MATN2, check out the MATN2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MATN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Matrilin-2 ELISA Kit PicoKine® (EK2020)
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Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human Matrilin-2 ELISA Kit PicoKine®
Question
Q: how do I analyze ELISA data? I measured Matrilin-2 level in serum.
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-01-29
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
G. King
Verified customer
Asked: 2019-12-31
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-31
Question
Q: how can I thaw whole blood sample for Matrilin-2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-11-10
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Matrilin-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-11-10
Question
Q: Can Matrilin-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
B. Brown
Verified customer
Asked: 2018-09-15
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-09-15
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-07-04
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-07-04
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Wu
Verified customer
Asked: 2018-02-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-02-12
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Matrilin-2 using the EK2020 Human Matrilin-2 Picokine® ELISA Kit?
C. Carter
Verified customer
Asked: 2014-08-21
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2014-08-21
