Click image to see more details
Product Info Summary
| SKU: | EK1687 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human MADCAM-1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1687
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MADCAM-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human MADCAM1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MADCAM-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1687)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q19-Q317
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1687 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1687 is reactive to MADCAM1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1687 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MAdCAM-1
MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al (1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4/beta7), L-selectin, and VLA-4 (alpha4/beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1687-CAP | Anti-Human MADCAM1 Pre-coated 96-well strip microplate | 1 |
| EK1687-ST | Human MADCAM1 Standard | 2 vials, 10 ng/tube |
| EK1687-DA | Human MADCAM1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.026 | 0.11 | 0.178 | 0.359 | 0.659 | 1.062 | 1.428 | 1.994 |
Data Example Images
Click image to see more details
Boster Kit Box
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MAdCAM-1, Dilution ratio of 1:5, concentration in serum and plasma is 1.2-2.2 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MADCAM-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 72 | 241 | 1030 | 77 | 233 | 996 |
| Standard deviation | 4.96 | 16.5%6 | 62.83 | 6.1%2 | 16.07 | 62.74 |
| CV (%) | 6.9% | 6.5% | 6.1% | 7.7% | 6.9% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MAdCAM-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 72 | 73 | 78 | 81 | 76 | 3.67 | 4.8% |
| Sample 2 | 241 | 227 | 222 | 222 | 228 | 7.77 | 3.4% |
| Sample 3 | 1030 | 1091 | 1082 | 1036 | 1059 | 27.02 | 2.5% |
Protein Target Info & Infographic
Gene/Protein Information For MADCAM1 (Source: Uniprot.Org, NCBI)
Gene Name
MADCAM1
Full Name
Mucosal addressin cell adhesion molecule 1
Weight
40.155kDa
Alternative Names
Mucosal addressin cell adhesion molecule 1; MAdCAM-1; hMAdCAM-1; MADCAM1 MADCAM1 MACAM1 mucosal vascular addressin cell adhesion molecule 1 mucosal addressin cell adhesion molecule 1|MAdCAM-1|hMAdCAM-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MADCAM1, check out the MADCAM1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MADCAM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MADCAM-1 ELISA Kit PicoKine® (EK1687)
Hello CJ!
No publications found for EK1687
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human MADCAM-1 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human MADCAM-1 ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Human MADCAM-1 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Mehta
Verified customer
Asked: 2019-04-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-04-30
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement MAdCAM-1 using the EK1687 Human MAdCAM-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-11-25
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-11-25
Question
Q: What is the optimal O.D. value for MAdCAM-1 ELISA kit? I used your MAdCAM-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain MAdCAM-1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MAdCAM-1 ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of MAdCAM-1 that can be declared/interpreted as positive by the above standard is 10pg/ml .
Boster Scientific Support
Answered: 2018-03-22
Question
Q: which procedure should I follow in order to thaw whole blood sample for MAdCAM-1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-02-17
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MAdCAM-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-02-17
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2016-09-07
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-09-07
