Human IL-38/IL1F10 ELISA Kit PicoKine®

IL-38/IL-1F10 ELISA kit for Human

Human IL-38/IL1F10 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL1F10 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays. Cited in 1 publication(s).

Product Info Summary

SKU: EK1662
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human IL-38/IL1F10 ELISA Kit PicoKine®

View all IL-38/IL-1F10 ELISA kits

SKU/Catalog Number

EK1662

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-38/IL1F10 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL1F10 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human IL-38/IL1F10 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1662)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: M1-W152

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK1662 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1662 is reactive to IL1F10 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1662 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-38/IL-1F10

Interleukin-1 family member 10 is a protein that in humans is encoded by the IL1F10 gene. The protein encoded by thi s gene is a member of the interleukin 1 cytokine family. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.

Kit Components

Catalog Number Description Quantity
EK1662-CAP Anti-Human IL1F10 Pre-coated 96-well strip microplate 1
EK1662-ST Human IL1F10 Standard 2 vials, 10 ng/tube
EK1662-DA Human IL1F10 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0720.1370.2180.3860.6461.0881.6382.230

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-38/IL-1F10, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 62.5 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-38/IL1F10 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)20344919841974831980
Standard deviation12.5826.9487.2913.9838.1599
CV (%)6.2%6%4.4%7.1%7.9%5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-38/IL-1F10 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 120321520617720014.137%
Sample 24494564554644565.331.1%
Sample 31984203818851780192198.535.1%
*number of samples for each test n=16.

Gene/Protein Information For IL1F10 (Source: Uniprot.Org, NCBI)

Gene Name

IL1F10

Full Name

Interleukin-1 family member 10

Weight

16.943kDa

Superfamily

IL-1 family

Alternative Names

Interleukin-1 family member 10; IL-1F10; Family of interleukin 1-theta; FIL1 theta; Interleukin-1 HY2; IL-1HY2; Interleukin-1 theta; IL-1 theta; Interleukin-38; IL-38; IL1F10; FIL1T; IL1HY2; IL38; FKSG75; UNQ6119/PRO20041 IL1F10 FIL1-theta, FKSG75, IL-1HY2, IL-38, IL1-theta, IL1HY2 interleukin 1 family member 10 interleukin-1 family member 10|FIL1 theta|IL-1 theta|IL-1F10 (canonical form IL-1F10a)|family of interleukin 1-theta|interleukin 1 family member 10 (theta)|interleukin-1 HY2|interleukin-1 receptor antagonist FKSG75|interleukin-1 receptor antagonist-like FIL1 theta|interleukin-38

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL1F10, check out the IL1F10 Infographic

IL1F10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL1F10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK1662 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Abassifard M, Khorramdelazad H, Rezaee S, Jafarzadeh A. Higher Circulating Concentration of Interleukin-38 in Patients with Knee Osteoarthritis: Its Association with Disease Severity. Iran J Allergy Asthma Immunol. :1-6.
Species: Human
EK1662 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

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6 Customer Q&As for Human IL-38/IL1F10 ELISA Kit PicoKine®

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IL-38 using the EK1662 Human IL-38 Picokine® ELISA Kit?

E. Zhao

Verified customer

Asked: 2020-07-09

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2020-07-09

Question

Q: if the enzyme conjugated IL-38 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-38 antigen?

R. Dhawan

Verified customer

Asked: 2019-12-06

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-12-06

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-03-03

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-03-03

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-11-23

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-11-23

Question

Q: can you suggest the dilution ratio of serum samples for detection of IL-38 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Z. Varadkar

Verified customer

Asked: 2018-05-30

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-38 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-05-30

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2016-10-21

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2016-10-21

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