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Product Info Summary
| SKU: | EK2205 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human GGCT/CRF21 ELISA Kit PicoKine®
View all gamma-Glutamylcyclotransferase/CRF21/GGCT ELISA kits
SKU/Catalog Number
EK2205
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human GGCT/CRF21 Kit PicoKine® (96 Tests). Quantitate Human GGCT in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human GGCT/CRF21 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2205)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: M1-L188
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2205 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2205 is reactive to GGCT in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2205 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of gamma-Glutamylcyclotransferase/CRF21/GGCT
The protein encoded by this gene catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism, and may play a critical role in glutathione homeostasis. The encoded protein may also play a role in cell proliferation, and the expression of this gene is a potential marker for cancer. Pseudogenes of this gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 20. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2205-CAP | Anti-Human GGCT Pre-coated 96-well strip microplate | 1 |
| EK2205-ST | Human GGCT Standard | 2 vials, 10 ng/tube |
| EK2205-DA | Human GGCT Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
| O.D. | 0.031 | 0.061 | 0.119 | 0.178 | 0.408 | 0.689 | 1.348 | 2.051 |
Data Example Images
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Human GGCT PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human GGCT/CRF21 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 181 | 452 | 841 | 223 | 489 | 892 |
| Standard deviation | 9.59 | 23.05 | 49.62 | 10.04 | 19.07 | 41.92 |
| CV (%) | 5.3% | 5.1% | 5.9% | 4.5% | 3.9% | 4.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of gamma-Glutamylcyclotransferase/CRF21/GGCT in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 181 | 179 | 168 | 202 | 183 | 8.76 | 4.8% |
| Sample 2 | 452 | 469 | 448 | 486 | 464 | 24.58 | 5.3% |
| Sample 3 | 841 | 869 | 851 | 883 | 861 | 47.36 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For GGCT (Source: Uniprot.Org, NCBI)
Gene Name
GGCT
Full Name
Gamma-glutamylcyclotransferase
Weight
21.008kDa
Superfamily
gamma-glutamylcyclotransferase family
Alternative Names
C7orf24; CRF21; gammaGlutamylcyclotransferase; gamma-Glutamylcyclotransferase; GCTG; GGC; GGCT; LOC79017; MGC3077 GGCT C7orf24, CRF21, GCTG, GGC gamma-glutamylcyclotransferase gamma-glutamylcyclotransferase|cytochrome c-releasing factor 21|gamma -glutamyl cyclotransferase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GGCT, check out the GGCT Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GGCT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human GGCT/CRF21 ELISA Kit PicoKine® (EK2205)
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