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Product Info Summary
| SKU: | EK0360 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human G-CSF ELISA Kit PicoKine®
SKU/Catalog Number
EK0360
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human G-CSF ELISA Kit PicoKine® (96 Tests). Quantitate Human CSF3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human G-CSF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0360)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: T31-P204
Sensitivity
<4 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0360 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0360 is reactive to CSF3 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0360 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of G-CSF
Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0360-CAP | Anti-Human CSF3 Pre-coated 96-well strip microplate | 1 |
| EK0360-ST | Human CSF3 Standard | 2 vials, 10 ng/tube |
| EK0360-DA | Human CSF3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.001 | 0.052 | 0.105 | 0.187 | 0.435 | 0.746 | 1.475 | 1.817 |
Data Example Images
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Human G-CSF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human G-CSF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 62 | 237 | 1001 | 64 | 230 | 966 |
| Standard deviation | 3.96 | 16.26 | 45.04 | 4.99 | 18.17 | 59.89 |
| CV (%) | 6.4% | 6.4% | 4.5% | 7.8% | 7.9% | 6.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of G-CSF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 62 | 57 | 60 | 56 | 58 | 2.38 | 4.1% |
| Sample 2 | 237 | 209 | 236 | 225 | 226 | 11.27 | 4.9% |
| Sample 3 | 1001 | 964 | 888 | 983 | 959 | 43.02 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For CSF3 (Source: Uniprot.Org, NCBI)
Gene Name
CSF3
Full Name
Granulocyte colony-stimulating factor
Weight
22293 MW
Superfamily
IL-6 superfamily
Alternative Names
Granulocyte colony-stimulating factor;G-CSF;Pluripoietin;Filgrastim;Lenograstim;CSF3;C17orf33, GCSF; CSF3 C17orf33OS, GCSF, CSF3 colony stimulating factor 3 granulocyte colony-stimulating factor|filgrastim|granulocyte-colony stimulating factor|lenograstim|pluripoietin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CSF3, check out the CSF3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CSF3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human G-CSF ELISA Kit PicoKine® (EK0360)
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Human G-CSF ELISA Kit PicoKine®
Question
Q: Can G-CSF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-09-17
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-09-17
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
T. Smith
Verified customer
Asked: 2020-07-21
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-07-21
Question
Q: if the enzyme conjugated G-CSF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the G-CSF antigen?
Verified Customer
Verified customer
Asked: 2020-05-18
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-05-18
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-07-31
Question
Q: which procedure should I follow in order to thaw whole blood sample for G-CSF ELISA after freezing?
Z. Wang
Verified customer
Asked: 2019-03-12
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test G-CSF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-03-12
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement G-CSF using the EK0360 Human G-CSF Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-02-13
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-02-13
Question
Per customer request, can you please provide the following information for EK0360? 1)What is the host of the capture antibody? 2)What is the host of the detection antibody? 3)Is the standard a recombinant protein? If yes, what is the host species?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
1) What is the host of the capture antibody? Mouse 2) What is the host of the detection antibody? Goat 3) Is the standard a recombinant protein? If yes, what is the host species? The standard is a recombinant protein. The expression system is E.coli.
Boster Scientific Support
Answered: 2019-01-02
Question
Does this kit cross-react with murine species?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
EK0360 does not cross-react with murine species.
Boster Scientific Support
Answered: 2018-12-10
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-01-05
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2016-09-08
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-09-08
Question
Will this kit detect PEGylated Human G-CSF? Keyword: detection, applications
Verified Customer
Verified customer
Asked: 2015-03-11
Answer
Human G-CSF ELISA kit can detect PEGylated Human G-CSF.
Boster Scientific Support
Answered: 2015-03-11
