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Product Info Summary
| SKU: | EK1242 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. |
Product info
Product Name
Human FOLR1/Alpha Fr ELISA Kit PicoKine®
SKU/Catalog Number
EK1242
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FOLR1/Alpha Fr ELISA Kit PicoKine® (96 Tests). Quantitate Human FOLR1 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FOLR1/Alpha Fr ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1242)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: R25-S234
Sensitivity
<10 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1242 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1242 is reactive to FOLR1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
Application Guarantee
EK1242 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FOLR1
Folate receptor 1 adult is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family. It is mapped to 11q13.4. FOLR1 expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The FOLR1 gene encodes the adult folate receptor, or folate-binding protein (FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is also an important regulator of milk protein synthesis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1242-CAP | Anti-Human FOLR1 Pre-coated 96-well strip microplate | 1 |
| EK1242-ST | Human FOLR1 Standard | 2 vials, 10 ng/tube |
| EK1242-DA | Human FOLR1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 46.9 | 94 | 188 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.042 | 0.072 | 0.135 | 0.222 | 0.359 | 0.674 | 1.227 | 1.978 |
Data Example Images
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Human FOLR1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FOLR1, Dilution ratio of 1:1, concentration in serum and plasma is around 300 pg/ml..
Some articles we found to cite concentrations of FOLR1 in samples: 23528302 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FOLR1/Alpha Fr ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 103 | 333 | 1306 | 108 | 312 | 1388 |
| Standard deviation | 7.41 | 22.31 | 88.8 | 8.53 | 24.96 | 119.36 |
| CV (%) | 7.2% | 6.7% | 6.8% | 7.9% | 8% | 8.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FOLR1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 103 | 108 | 100 | 99 | 102 | 3.5 | 3.4% |
| Sample 2 | 333 | 359 | 355 | 339 | 346 | 10.8 | 3.1% |
| Sample 3 | 1306 | 1386 | 1411 | 1506 | 1402 | 71.35 | 5% |
Protein Target Info & Infographic
Gene/Protein Information For FOLR1 (Source: Uniprot.Org, NCBI)
Gene Name
FOLR1
Full Name
Folate receptor alpha
Weight
29819 MW
Superfamily
folate receptor family
Alternative Names
Folate receptor alpha;FR-alpha;Adult folate-binding protein;FBP;Folate receptor 1;Folate receptor, adult;KB cells FBP;Ovarian tumor-associated antigen MOv18;FOLR1;FOLR; FOLR1 FBP, FOLR, FRalpha, NCFTD folate receptor alpha folate receptor alpha|FR-alpha|KB cells FBP|adult folate-binding protein|folate binding protein|folate receptor 1 (adult)|folate receptor, adult|ovarian tumor-associated MOv18
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FOLR1, check out the FOLR1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FOLR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human FOLR1/Alpha Fr ELISA Kit PicoKine® (EK1242)
Hello CJ!
EK1242 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Folate-receptor 1 level in periodontal disease: a pilot study
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human FOLR1/Alpha Fr ELISA Kit PicoKine®
Question
Q: is it okay to use citrate plasma as samples in Human FOLR1 Picokine® ELISA Kit (Catalog # EK1242)?
G. Bhasin
Verified customer
Asked: 2020-01-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of FOLR1 causing degradation of its protein structure. FOLR1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the FOLR1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-01-12
Question
Q: how can I thaw whole blood sample for FOLR1 ELISA after freezing?
T. Young
Verified customer
Asked: 2019-08-01
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FOLR1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-08-01
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of FOLR1 in Human urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
V. Parker
Verified customer
Asked: 2018-12-16
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FOLR1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-12-16
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-11-01
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-11-01
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-10-25
Question
Q: if the enzyme conjugated FOLR1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FOLR1 antigen?
Verified Customer
Verified customer
Asked: 2016-04-01
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-04-01
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
P. Parker
Verified customer
Asked: 2016-01-01
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-01-01
