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Product Info Summary
| SKU: | EK0545 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human FLT4/VEGFR3 ELISA Kit PicoKine®
View all VEGFR3/Flt-4 ELISA kits
SKU/Catalog Number
EK0545
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FLT4/VEGFR3 ELISA Kit PicoKine® (96 Tests). Quantitate Human FLT4 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FLT4/VEGFR3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0545)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Y25-I776
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0545 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0545 is reactive to FLT4 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0545 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VEGFR3/Flt-4
Fms-related tyrosine kinase 4, also known as FLT4, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0545-CAP | Anti-Human FLT4 Pre-coated 96-well strip microplate | 1 |
| EK0545-ST | Human FLT4 Standard | 2 vials, 10 ng/tube |
| EK0545-DA | Human FLT4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.057 | 0.101 | 0.156 | 0.256 | 0.456 | 0.896 | 1.592 | 2.256 |
Data Example Images
Click image to see more details
Human FLT4/VEGFR3 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FLT4/VEGFR3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 268 | 670 | 2924 | 294 | 854 | 3861 |
| Standard deviation | 10.99 | 28.81 | 134.50 | 14.99 | 50.39 | 258.69 |
| CV (%) | 4.1% | 4.3% | 4.6% | 5.1% | 5.9% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of VEGFR3/Flt-4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 268 | 282 | 273 | 286 | 277 | 7.11 | 2.5% |
| Sample 2 | 670 | 653 | 609 | 610 | 635 | 26.68 | 4.2% |
| Sample 3 | 2924 | 3021 | 2641 | 3116 | 2925 | 177.73 | 6.0% |
Protein Target Info & Infographic
Gene/Protein Information For FLT4 (Source: Uniprot.Org, NCBI)
Gene Name
FLT4
Full Name
Vascular endothelial growth factor receptor 3
Weight
152.757kDa
Superfamily
protein kinase superfamily
Alternative Names
Vascular endothelial growth factor receptor 3; VEGFR-3; Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4; FLT4; VEGFR3 FLT4 CHTD7, FLT-41, LMPH1A, LMPHM1, PCL, VEGFR-3, VEGFR3, FLT4 fms related receptor tyrosine kinase 4 vascular endothelial growth factor receptor 3|Feline McDonough Sarcoma (FMS)-like tyrosine kinase 4|VEGF receptor-3|fms related tyrosine kinase 4|fms-like tyrosine kinase 4|primary congenital lymphedema|tyrosine-protein kinase receptor FLT4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FLT4, check out the FLT4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FLT4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human FLT4/VEGFR3 ELISA Kit PicoKine® (EK0545)
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human FLT4/VEGFR3 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Walker
Verified customer
Asked: 2020-11-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-11-24
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
E. Roberts
Verified customer
Asked: 2020-03-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-03-22
Question
Q: how do I analyze ELISA data? I have performed VEGFR3 level in serum.
Verified Customer
Verified customer
Asked: 2018-12-22
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-12-22
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of VEGFR3 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the VEGFR3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-07-02
Question
Q: if the enzyme conjugated VEGFR3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the VEGFR3 antigen?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-01-25
Question
Q: Can VEGFR3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
A. Babu
Verified customer
Asked: 2017-09-17
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2017-09-17
Question
Q: how to thaw whole blood sample for VEGFR3 ELISA after freezing?
H. Chen
Verified customer
Asked: 2016-05-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test VEGFR3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-05-20
Question
Q: is it okay to use citrate plasma as samples in Human VEGFR3 Picokine® ELISA Kit (Catalog # EK0545)?
A. Mitchell
Verified customer
Asked: 2015-04-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of VEGFR3 causing disruption of its protein structure. VEGFR3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the VEGFR3 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-04-28
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement VEGFR3 using the EK0545 Human VEGFR3 Picokine® ELISA Kit?
A. Harris
Verified customer
Asked: 2014-10-29
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2014-10-29
