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Product Info Summary
| SKU: | EK0352 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, lysates, tissue, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human FLT3/FLK2 ELISA Kit PicoKine®
View all Flt-3/Flk-2/CD135 ELISA kits
SKU/Catalog Number
EK0352
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FLT3/FLK2 ELISA Kit PicoKine® (96 Tests). Quantitate Human FLT3 in cell culture supernatants, lysates, tissue, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FLT3/FLK2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0352)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: N27-N541
Sensitivity
<50 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0352 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0352 is reactive to FLT3 in Human samples
Validated Sample Types
cell culture supernatants, lysates, tissue, serum and plasma (heparin, EDTA).
Application Guarantee
EK0352 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Flt-3/Flk-2/CD135
Cluster of differentiation antigen 135 (CD135) also known as fms like tyrosine kinase 3 (FLT-3), receptor-type tyrosine-protein kinase FLT3, or fetal liver kinase-2 (Flk2) is a protein that in humans is encoded by the FLT3 gene. It is mapped to 13q12.2. This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0352-CAP | Anti-Human FLT3 Pre-coated 96-well strip microplate | 1 |
| EK0352-ST | Human FLT3 Standard | 2 vials, 10 ng/tube |
| EK0352-DA | Human FLT3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.025 | 0.072 | 0.117 | 0.200 | 0.414 | 0.688 | 1.221 | 2.008 |
Data Example Images
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Human FLT3/FLK2 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Flt-3/Flk-2/CD135, Dilution ratio of 1:1, concentration in serum and plasma is around 100 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FLT3/FLK2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 73 | 300 | 1365 | 82 | 381 | 1527 |
| Standard deviation | 3.50 | 14.70 | 64.16 | 5.41 | 24.38 | 105.36 |
| CV (%) | 4.8% | 4.9% | 4.7% | 6.6% | 6.4% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Flt-3/Flk-2/CD135 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 73 | 72 | 68 | 77 | 72 | 3.20 | 4.4% |
| Sample 2 | 300 | 276 | 286 | 285 | 286 | 8.58 | 3.0% |
| Sample 3 | 1365 | 1410 | 1476 | 1562 | 1453 | 74.16 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For FLT3 (Source: Uniprot.Org, NCBI)
Gene Name
FLT3
Full Name
Receptor-type tyrosine-protein kinase FLT3
Weight
112.903kDa
Superfamily
protein kinase superfamily
Alternative Names
Receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; Fetal liver kinase-2; FLK-2; Fms-like tyrosine kinase 3; FLT-3; Stem cell tyrosine kinase 1; STK-1; CD135; FLT3; CD135; FLK2; STK1 FLT3 CD135, FLK-2, FLK2, STK1 fms related receptor tyrosine kinase 3 receptor-type tyrosine-protein kinase FLT3|CD135 |FL cytokine receptor|fetal liver kinase 2|fms related tyrosine kinase 3|fms-like tyrosine kinase 3|growth factor receptor tyrosine kinase type III|stem cell tyrosine kinase 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FLT3, check out the FLT3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FLT3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human FLT3/FLK2 ELISA Kit PicoKine® (EK0352)
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No publications found for EK0352
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human FLT3/FLK2 ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human CD135 Picokine® ELISA Kit (Catalog # EK0352)?
Verified Customer
Verified customer
Asked: 2020-06-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CD135 causing disruption of its protein structure. CD135 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CD135 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-06-04
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
K. Varma
Verified customer
Asked: 2020-01-06
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-01-06
Question
Q: Can CD135 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-08-17
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-08-17
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Zhang
Verified customer
Asked: 2019-06-25
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-06-25
Question
Q: if the enzyme conjugated CD135 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD135 antigen?
W. Williams
Verified customer
Asked: 2018-07-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-07-23
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CD135 in Human tissue? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
H. Jha
Verified customer
Asked: 2014-09-28
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD135 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2014-09-28
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CD135 using the EK0352 Human CD135 Picokine® ELISA Kit?
S. Dhawan
Verified customer
Asked: 2014-08-30
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2014-08-30
