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Product Info Summary
| SKU: | A00188-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Flt3/CD135 Antibody Picoband®
View all Flt-3/Flk-2/CD135 Antibodies
SKU/Catalog Number
A00188-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Flt3/CD135 Antibody Picoband® catalog # A00188-4. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Flt3/CD135 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00188-4)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Flt3 / CD135 recombinant protein (Position: E62-E295).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00188-4 is reactive to Flt3 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
160 kDa
Calculated molecular weight
112.903kDa
Background of Flt-3/Flk-2/CD135
Cluster of differentiation antigen 135 (CD135) also known as fms like tyrosine kinase 3 (FLT-3), receptor-type tyrosine-protein kinase FLT3, or fetal liver kinase-2 (Flk2) is a protein that in humans is encoded by the FLT3 gene. This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00188-4 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse brain tissue
IHC: mouse small intestine tissue, rat kidney tissue, rat liver tissue, rat small intestine tissue, mouse kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (A00188-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flt3 / CD135 antigen affinity purified polyclonal antibody (Catalog # A00188-4) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Flt3 / CD135 at approximately 160KD. The expected band size for Flt3 / CD135 is at 113KD.
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Figure 2. IHC analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (A00188-4).
Flt3 / CD135 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Flt3 / CD135 Antibody (A00188-4) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (A00188-4).
Flt3 / CD135 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Flt3 / CD135 Antibody (A00188-4) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (A00188-4).
Flt3 / CD135 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Flt3 / CD135 Antibody (A00188-4) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (A00188-4).
Flt3 / CD135 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Flt3 / CD135 Antibody (A00188-4) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (A00188-4).
Flt3 / CD135 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Flt3 / CD135 Antibody (A00188-4) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Flt3 (Source: Uniprot.org, NCBI)
Gene Name
Flt3
Full Name
Receptor-type tyrosine-protein kinase FLT3
Weight
112.903kDa
Superfamily
protein kinase superfamily
Alternative Names
Receptor-type tyrosine-protein kinase FLT3; 2.7.10.1; FL cytokine receptor; Fetal liver kinase 2; FLK-2; Fms-like tyrosine kinase 3; FLT-3; Tyrosine-protein kinase receptor flk-2; CD135; Flt3; Flk-2, Flt-3; FLT3 CD135, FLK-2, FLK2, STK1 fms related receptor tyrosine kinase 3 receptor-type tyrosine-protein kinase FLT3|CD135 |FL cytokine receptor|fetal liver kinase 2|fms related tyrosine kinase 3|fms-like tyrosine kinase 3|growth factor receptor tyrosine kinase type III|stem cell tyrosine kinase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Flt3, check out the Flt3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Flt3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Flt3/CD135 Antibody Picoband® (A00188-4)
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Customer Q&As
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4 Customer Q&As for Anti-Flt3/CD135 Antibody Picoband®
Question
Our team were satisfied with the WB result of your anti-Flt3/CD135 antibody. However we have seen positive staining in lymphocyte membrane using this antibody. Is that expected? Could you tell me where is FLT3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
According to literature, lymphocyte does express FLT3. Generally FLT3 expresses in membrane. Regarding which tissues have FLT3 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7507245
Lymphocyte, Pubmed ID: 8394751, 15057823
Testis, Pubmed ID: 2004790
Boster Scientific Support
Answered: 2020-03-20
Question
We purchased anti-Flt3/CD135 antibody for ELISA on lymphocyte in a previous experiment. I am using mouse, and I plan to use the antibody for WB next. I am interested in examining lymphocyte as well as bone marrow in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
I took a look at the website and datasheets of our anti-Flt3/CD135 antibody and it appears that A00188-4 has been tested on mouse in both ELISA and WB. Thus A00188-4 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-22
Question
We have observed staining in mouse cerebellar hemisphere. Do you have any suggestions? Is anti-Flt3/CD135 antibody supposed to stain cerebellar hemisphere positively?
K. Williams
Verified customer
Asked: 2017-05-22
Answer
From what I have seen in literature cerebellar hemisphere does express FLT3. From what I have seen in Uniprot.org, FLT3 is expressed in cerebellar hemisphere, bone marrow, lymphocyte, testis, among other tissues. Regarding which tissues have FLT3 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 7507245
Lymphocyte, Pubmed ID: 8394751, 15057823
Testis, Pubmed ID: 2004790
Boster Scientific Support
Answered: 2017-05-22
Question
We are currently using anti-Flt3/CD135 antibody A00188-4 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on pig tissues as well?
V. Parker
Verified customer
Asked: 2013-03-18
Answer
The anti-Flt3/CD135 antibody (A00188-4) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-18
