Human F7 ELISA Kit PicoKine®

Product Name

Human F7 ELISA Kit PicoKine®

View all Coagulation Factor VII ELISA kits

SKU/Catalog Number

EK2197

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human F7 ELISA Kit PicoKine® (96 Tests). Quantitate Human F7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human F7 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2197)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: A39-P444

Sensitivity

<20 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Standard Dilution Instructions

See datasheet of EK2197 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2197 is reactive to F7 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2197 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Coagulation Factor VII

Coagulation factor VII (EC 3.4.21.21, formerly known as proconvertin) is one of the proteins that causes blood to clot in the coagulation cascade, and in humans is coded for by the gene F7. This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.

Kit Components

Catalog Number	Description	Quantity
EK2197-CAP	Anti-Human F7 Pre-coated 96-well strip microplate	1
EK2197-ST	Human F7 Standard	2 vials, 50 ng/tube
EK2197-DA	Human F7 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.007	0.073	0.124	0.272	0.582	0.988	1.342	2.022

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human F7 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Coagulation Factor VII in ELISA kits from four different production batches/lots.

Gene/Protein Information For F7 (Source: Uniprot.Org, NCBI)

Alternative Names

coagulation factor VII (serum prothrombin conversion accelerator); Coagulation Factor VII; EC 3.4.21; EC 3.4.21.21; Eptacog alfa; F7; FVII coagulation protein; proconvertin; Serum prothrombin conversion accelerator; SPCA F7 SPCA coagulation factor VII coagulation factor VII|FVII coagulation protein|coagulation factor VII (serum prothrombin conversion accelerator)|eptacog alfa|proconvertin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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