Product Info Summary
SKU: | EK2187 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human DSC2 ELISA Kit PicoKine®
View all Desmocollin-2 ELISA kits
SKU/Catalog Number
EK2187
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human DSC2 ELISA Kit PicoKine® (96 Tests). Quantitate Human DSC2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human DSC2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2187)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: R136-R684
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2187 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2187 is reactive to DSC2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2187 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Desmocollin-2
This gene encodes a member of the desmocollin protein subfamily. Desmocollins, along with desmogleins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmocollin family members on chromosome 18. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11, and reduced protein expression has been described in several types of cancer. Alternative splicing results in multiple transcript variants.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK2187-CAP | Anti-Human DSC2 Pre-coated 96-well strip microplate | 1 |
EK2187-ST | Human DSC2 Standard | 2 vials, 10 ng/tube |
EK2187-DA | Human DSC2 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
O.D. | 0.043 | 0.136 | 0.192 | 0.312 | 0.545 | 0.908 | 1.441 | 2.033 |
Data Example Images
![ek2187 ek2187](https://www.bosterbio.com/media/catalog/product/e/k/ek2187.png)
Click image to see more details
Human DSC2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human DSC2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 236 | 1364 | 5315 | 241 | 1354 | 5413 |
Standard deviation | 17.46 | 94.12 | 340.16 | 14.94 | 79.89 | 373.5 |
CV (%) | 7.4% | 6.9% | 6.4% | 6.2% | 5.9% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Desmocollin-2 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 236 | 198 | 216 | 223 | 218 | 11.13 | 5.1% |
Sample 2 | 1364 | 1352 | 1456 | 1316 | 1372 | 52.14 | 3.8% |
Sample 3 | 5315 | 5126 | 5241 | 5611 | 5323 | 218.25 | 4.1% |
Protein Target Info & Infographic
Gene/Protein Information For DSC2 (Source: Uniprot.Org, NCBI)
Gene Name
DSC2
Full Name
Desmocollin-2
Weight
Alternative Names
ARVD11; Cadherin family member 2; CDHF2; CDHF2DGII/III; desmocollin 2; Desmocollin2; Desmocollin-2; Desmocollin-3; Desmosomal glycoprotein II; desmosomal glycoprotein II/III; Desmosomal glycoprotein III; DG2; DSC2; DSC3DKFZp686I11137 DSC2 ARVD11, CDHF2, DG2, DGII/III, DSC3 desmocollin 2 desmocollin-2|cadherin family member 2|desmosomal glycoprotein II/III
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DSC2, check out the DSC2 Infographic
![DSC2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DSC2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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