Anti-Desmocollin-2/3 DSC2 Monoclonal Antibody

Product Name

Anti-Desmocollin-2/3 DSC2 Monoclonal Antibody

View all Desmocollin-2 Antibodies

SKU/Catalog Number

M03205

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Desmocollin-2/3 DSC2 Monoclonal Antibody (Catalog # M03205). Tested in Flow Cytometry, IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Desmocollin-2/3 DSC2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03205)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: 7G6

Isotype

IgG1, kappa

Immunogen

Human desmocollin-2 extracellular domain

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M03205 is reactive to DSC2 in Human, Mouse, Rat

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

99962 MW

Background of Desmocollin-2

Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. And desmocollin-3 is expressed more evenly throughout the suprabasal layers.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03205 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1.0ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For DSC2 (Source: Uniprot.org, NCBI)

Alternative Names

Desmocollin-2;Cadherin family member 2;Desmocollin-3;Desmosomal glycoprotein II;Desmosomal glycoprotein III;DSC2;CDHF2, DSC3; DSC2 ARVD11, CDHF2, DG2, DGII/III, DSC3 desmocollin 2 desmocollin-2|cadherin family member 2|desmosomal glycoprotein II/III

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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