Click image to see more details
Product Info Summary
| SKU: | EK1276 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , urine and human milk. |
Product info
Product Name
Human DLL1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1276
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human DLL1 ELISA Kit PicoKine® (96 Tests). Quantitate Human DLL1 in cell culture supernatants, serum, plasma (heparin, EDTA) , urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human DLL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1276)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S22-G540
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1276 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1276 is reactive to DLL1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , urine and human milk.
Application Guarantee
EK1276 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Dll1
DLL1 (DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1276-CAP | Anti-Human DLL1 Pre-coated 96-well strip microplate | 1 |
| EK1276-ST | Human DLL1 Standard | 2 vials, 10 ng/tube |
| EK1276-DA | Human DLL1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.003 | 0.232 | 0.373 | 0.705 | 1.012 | 1.459 | 1.811 | 1.902 |
Data Example Images
Click image to see more details
Human DLL1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Dll1, Dilution ratio of 1:5, concentration in serum is 830-2162 pg/ml..
Some articles we found to cite concentrations of Dll1 in samples: 21426637 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human DLL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 105 | 634 | 2353 | 102 | 662 | 2503 |
| Standard deviation | 4.62 | 27.26 | 94.12 | 4.69 | 31.11 | 137.66 |
| CV (%) | 4.4% | 4.3% | 4% | 4.6% | 4.7% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Dll1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 105 | 104 | 94 | 106 | 102 | 4.81 | 4.7% |
| Sample 2 | 634 | 578 | 595 | 596 | 600 | 20.48 | 3.4% |
| Sample 3 | 2353 | 2202 | 2459 | 2342 | 2339 | 91.34 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For DLL1 (Source: Uniprot.Org, NCBI)
Gene Name
DLL1
Full Name
Delta-like protein 1
Weight
78056 MW
Alternative Names
Delta-like protein 1;Drosophila Delta homolog 1;Delta1;H-Delta-1;DLL1;UNQ146/PRO172; Dll1|Delt, Delta1|delta like canonical Notch ligand 1|delta-like protein 1|delta like-1|delta-like 1|drosophila Delta homolog 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DLL1, check out the DLL1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DLL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human DLL1 ELISA Kit PicoKine® (EK1276)
Hello CJ!
No publications found for EK1276
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human DLL1 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human DLL1 ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Human DLL1 ELISA Kit PicoKine®
Question
Q: how do I analyze ELISA data? I measured Dll1 level in serum.
A. Adams
Verified customer
Asked: 2020-08-10
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-08-10
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
P. Miller
Verified customer
Asked: 2019-09-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-09-22
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-06-16
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-06-16
Question
Q: What is the optimal O.D. value for Dll1 ELISA kit? I performed your Dll1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Dll1 even though the O.D. values are not very high?
W. Babu
Verified customer
Asked: 2018-05-29
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Dll1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Dll1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-05-29
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Dll1 using the EK1276 Human Dll1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-03-24
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-03-24
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
S. Banerjee
Verified customer
Asked: 2014-09-01
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-09-01
