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Product Info Summary
| SKU: | EK2172 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CRTAM ELISA Kit PicoKine®
SKU/Catalog Number
EK2172
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CRTAM ELISA Kit PicoKine® (96 Tests). Quantitate Human CRTAM in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CRTAM ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2172)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S18-S286
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2172 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2172 is reactive to CRTAM in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2172 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Crtam
The CRTAM gene is upregulated in CD4-positive and CD8-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2172-CAP | Anti-Human CRTAM Pre-coated 96-well strip microplate | 1 |
| EK2172-ST | Human CRTAM Standard | 2 vials, 10 ng/tube |
| EK2172-DA | Human CRTAM Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.045 | 0.178 | 0.276 | 0.403 | 0.829 | 1.311 | 1.987 | 2.133 |
Data Example Images
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Human CRTAM PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CRTAM ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 53 | 284 | 914 | 51 | 322 | 942 |
| Standard deviation | 2.28 | 10.79 | 44.79 | 2.65 | 18.03 | 48.04 |
| CV (%) | 4.3% | 3.8% | 4.9% | 5.2% | 5.6% | 5.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Crtam in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 58 | 38 | 41 | 45 | 44 | 1.95 | 4.4% |
| Sample 2 | 284 | 285 | 271 | 266 | 277 | 8.85 | 3.2% |
| Sample 3 | 914 | 898 | 906 | 923 | 910 | 44.60 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For CRTAM (Source: Uniprot.Org, NCBI)
Gene Name
CRTAM
Full Name
Cytotoxic and regulatory T-cell molecule
Weight
43.907kDa
Superfamily
nectin family
Alternative Names
CD355 antigen; CD355; class I MHC restricted T cell associated molecule; class-I MHC-restricted T cell associated molecule; Class-I MHC-restricted T-cell-associated molecule; CRTAM; cytotoxic and regulatory T cell molecule; cytotoxic and regulatory T-cell molecule Crtam|cytotoxic and regulatory T cell molecule|cytotoxic and regulatory T-cell molecule|class I-restricted T cell-associated molecule|class-I MHC-restricted T-cell-associated molecule
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CRTAM, check out the CRTAM Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CRTAM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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