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Product Info Summary
| SKU: | EK1956 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CREG1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1956
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CREG1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CREG1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CREG1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1956)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: R32-Q220
Sensitivity
<50 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1956 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1956 is reactive to CREG1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1956 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CREG
This gene is mapped to 1q24.2. The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1956-CAP | Anti-Human CREG1 Pre-coated 96-well strip microplate | 1 |
| EK1956-ST | Human CREG1 Standard | 2 vials, 20 ng/tube |
| EK1956-DA | Human CREG1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.054 | 0.248 | 0.416 | 0.674 | 0.928 | 1.438 | 1.953 | 2.224 |
Data Example Images
Click image to see more details
Human CREG1 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CREG, Dilution ratio of 1:1, concentration in serum and plasma is around 6 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CREG1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 441 | 1500 | 8633 | 514 | 2089 | 9813 |
| Standard deviation | 21.61 | 79.50 | 578.41 | 32.90 | 100.27 | 578.97 |
| CV (%) | 4.9% | 5.3% | 6.7% | 6.4% | 4.8% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CREG in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 441 | 427 | 436 | 475 | 444 | 18.17 | 4.0% |
| Sample 2 | 1500 | 1602 | 1545 | 1419 | 1516 | 66.89 | 4.4% |
| Sample 3 | 8633 | 8533 | 7903 | 8527 | 8399 | 289.44 | 3.4% |
Protein Target Info & Infographic
Gene/Protein Information For CREG1 (Source: Uniprot.Org, NCBI)
Gene Name
CREG1
Full Name
Protein CREG1
Weight
24.075kDa
Superfamily
CREG family
Alternative Names
Protein CREG1; Cellular repressor of E1A-stimulated genes 1; CREG1; CREG; UNQ727/PRO1409 CREG1 CREG cellular repressor of E1A stimulated genes 1 protein CREG1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CREG1, check out the CREG1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CREG1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CREG1 ELISA Kit PicoKine® (EK1956)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human CREG1 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for CREG ELISA kit? I performed your CREG ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CREG even though the O.D. values are not very high?
W. Zhou
Verified customer
Asked: 2020-09-24
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CREG ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of CREG that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2020-09-24
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-08-30
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-30
Question
Q: can I use citrate plasma as samples in Human CREG Picokine® ELISA Kit (Catalog # EK1956)?
J. Edwards
Verified customer
Asked: 2020-04-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CREG causing degradation of its protein structure. CREG may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CREG ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-04-29
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CREG using the EK1956 Human CREG Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-04-26
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-04-26
Question
Q: which procedure should I follow in order to thaw whole blood sample for CREG ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CREG for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-11-28
Question
Q: how to analyze ELISA data? I have obtained CREG level in plasma.
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-06-02
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
W. Campbell
Verified customer
Asked: 2016-12-08
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-12-08
Question
Q: can you suggest the dilution ratio of serum samples for detection of CREG in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2016-08-06
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CREG ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-08-06
