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Product Info Summary
| SKU: | EK1953 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CHRDL1 ELISA Kit PicoKine®
View all Chordin-like 1/CHRDL1 ELISA kits
SKU/Catalog Number
EK1953
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CHRDL1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CHRDL1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CHRDL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1953)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E22-C450
Sensitivity
<50 pg/ml
Assay Range
93.6 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1953 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1953 is reactive to CHRDL1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1953 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Chordin-like 1/CHRDL1
Chordin-like 1 is a protein that in humans is encoded by the CHRDL1 gene. It is mapped to Xq23. This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1953-CAP | Anti-Human CHRDL1 Pre-coated 96-well strip microplate | 1 |
| EK1953-ST | Human CHRDL1 Standard | 2 vials, 10 ng/tube |
| EK1953-DA | Human CHRDL1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.088 | 0.134 | 0.176 | 0.265 | 0.402 | 0.702 | 1.263 | 2.099 |
Data Example Images
Click image to see more details
Human CHRDL1 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Chordin-like 1/CHRDL1, Dilution ratio of 1:1, concentration in serum and plasma is around 600 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CHRDL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 146 | 511 | 1796 | 201 | 618 | 2851 |
| Standard deviation | 7.59 | 27.08 | 100.58 | 13.07 | 41.41 | 196.72 |
| CV (%) | 5.2% | 5.3% | 5.6% | 6.5% | 6.7% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Chordin-like 1/CHRDL1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 146 | 138 | 126 | 133 | 135 | 7.29 | 5.4% |
| Sample 2 | 511 | 424 | 442 | 453 | 457 | 32.57 | 7.1% |
| Sample 3 | 1796 | 1945 | 1901 | 2041 | 1920 | 88.03 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For CHRDL1 (Source: Uniprot.Org, NCBI)
Gene Name
CHRDL1
Full Name
Chordin-like protein 1
Weight
50.732kDa
Alternative Names
Chordin-like protein 1; Neuralin-1; Neurogenesin-1; Ventroptin; CHRDL1; NRLN1 Chrdl1|CHL, CHL1, N, Nrln1, V, VOPT|chordin-like 1|chordin-like protein 1|neuralin-1|neurogenesin-1|ventroptin-alpha|ventroptin-beta
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CHRDL1, check out the CHRDL1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CHRDL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CHRDL1 ELISA Kit PicoKine® (EK1953)
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Recommended Resources
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human CHRDL1 ELISA Kit PicoKine®
Question
Q: how to thaw whole blood sample for Chordin-like 1 ELISA after freezing?
C. Kaur
Verified customer
Asked: 2020-03-06
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Chordin-like 1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-03-06
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Thomas
Verified customer
Asked: 2019-12-02
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-12-02
Question
Q: if the enzyme conjugated Chordin-like 1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Chordin-like 1 antigen?
L. Mangal
Verified customer
Asked: 2019-09-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-09-09
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-08-13
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Chordin-like 1 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-06-22
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Chordin-like 1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-06-22
Question
Q: What is the optimal O.D. value for Chordin-like 1 ELISA kit? I used your Chordin-like 1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Chordin-like 1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-01-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Chordin-like 1 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Chordin-like 1 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2019-01-15
Question
Q: can I use citrate plasma as samples in Human Chordin-like 1 Picokine® ELISA Kit (Catalog # EK1953)?
L. Lee
Verified customer
Asked: 2018-11-08
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Chordin-like 1 causing degradation of its protein structure. Chordin-like 1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Chordin-like 1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-11-08
Question
Q: how to proceed with the analysis of ELISA data? I measured Chordin-like 1 level in serum.
Verified Customer
Verified customer
Asked: 2017-09-24
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-09-24
