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Product Info Summary
| SKU: | EK1199 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum, plasma(heparin , EDTA) and urine. |
Product info
Product Name
Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®
View all Complement Factor D/Adipsin ELISA kits
SKU/Catalog Number
EK1199
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CFD/Adipsin ELISA Kit PicoKine® (96 Tests). Quantitate Human CFD in cell culture supernatants, cell lysates, serum, plasma(heparin , EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1199)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: I26-A253
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1199 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1199 is reactive to CFD in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum, plasma(heparin , EDTA) and urine.
Application Guarantee
EK1199 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Complement Factor D/Adipsin
Complement factor D (CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1199-CAP | Anti-Human CFD Pre-coated 96-well strip microplate | 1 |
| EK1199-ST | Human CFD Standard | 2 vials, 20 ng/tube |
| EK1199-DA | Human CFD Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 200000000 | 20000000 | 2000000 | 200000 | 20000 |
| O.D. | 0.009 | 0.093 | 0.182 | 0.358 | 0.701 | 1.156 | 1.822 | 2.355 |
Data Example Images
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Human CFD PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Complement Factor D/Adipsin, Dilution ratio of 1:1000, concentration in serum and plasma is 1382-3885 ng/ml..
Some articles we found to cite concentrations of Complement Factor D/Adipsin in samples: 24009749 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 787 | 2378 | 90804 | 804 | 2193 | 8576 |
| Standard deviation | 44.07 | 149.81 | 436.56 | 49.84 | 144.73 | 471.68 |
| CV (%) | 6.6%%% | 6.6%% | 4.8% | 6.2% | 6.6% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Complement Factor D/Adipsin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 787 | 751 | 817 | 732 | 771 | 32.75 | 4.2% |
| Sample 2 | 2378 | 2440 | 2261 | 2414 | 2373 | 68.44 | 2.8% |
| Sample 3 | 9095 | 10188 | 9364 | 8739 | 9346 | 534.02 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For CFD (Source: Uniprot.Org, NCBI)
Gene Name
CFD
Full Name
Complement factor D
Weight
27033 MW
Superfamily
peptidase S1 family
Alternative Names
Complement factor D;3.4.21.46;Adipsin;C3 convertase activator;Properdin factor D;CFD;DF, PFD; CFD ADIPSIN, ADN, DF, PFD complement factor D complement factor D|C3 convertase activator|D component of complement (adipsin)|complement factor D preproprotein|properdin factor D
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CFD, check out the CFD Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CFD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine® (EK1199)
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No publications found for EK1199
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Complement Factor D antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Complement Factor D antigen?
M. Lee
Verified customer
Asked: 2020-12-13
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-12-13
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-02-26
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
K. Scott
Verified customer
Asked: 2019-12-22
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-22
Question
Q: how to thaw whole blood sample for Complement Factor D ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-08-18
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Complement Factor D for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-08-18
Question
Q: how to analyze ELISA data? I have obtained Complement Factor D level in serum.
D. Zhang
Verified customer
Asked: 2018-10-07
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-10-07
Question
Q: can I use citrate plasma as samples in Human Complement Factor D Picokine® ELISA Kit (Catalog # EK1199)?
Verified Customer
Verified customer
Asked: 2018-09-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Complement Factor D causing disruption of its protein structure. Complement Factor D may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Complement Factor D ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-09-16
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Complement Factor D using the EK1199 Human Complement Factor D Picokine® ELISA Kit?
B. Dhawan
Verified customer
Asked: 2014-08-24
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2014-08-24
Question
Q: can you recommend the dilution ratio of serum samples for detection of Complement Factor D in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2014-04-12
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Complement Factor D ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2014-04-12
