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Product Info Summary
| SKU: | EK1668 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human CDCP1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1668
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CDCP1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CDCP1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CDCP1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1668)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: F30-T667
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1668 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1668 is reactive to CDCP1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK1668 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CDCP1
CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene. It has also been designated as CD318 (cluster of differentiation 318) and Trask (Transmembrane and associated with src kinases). CDCP1/Trask is a 140 kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD) containing five tyrosines. The tyrosine phosphorylation of Trask is tightly regulated and reciprocally linked with the state of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsin or EDTA, or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype. CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1668-CAP | Anti-Human CDCP1 Pre-coated 96-well strip microplate | 1 |
| EK1668-ST | Human CDCP1 Standard | 2 vials, 2 ng/tube |
| EK1668-DA | Human CDCP1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.042 | 0.142 | 0.208 | 0.363 | 0.67 | 1.061 | 1.413 | 2.093 |
Data Example Images
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Human CDCP1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CDCP1, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CDCP1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 54 | 307 | 865 | 54 | 291 | 945 |
| Standard deviation | 4.26 | 12.89 | 62.28 | 4.59 | 14.84 | 68.98 |
| CV (%) | 7.9% | 4.2% | 7.2% | 8.5% | 7.3%% | 7.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CDCP1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 54 | 53 | 62 | 62 | 57 | 4.26 | 7.4% |
| Sample 2 | 307 | 305 | 306 | 280 | 299 | 11.28 | 3.7% |
| Sample 3 | 865 | 848 | 944 | 941 | 899 | 43.43 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For CDCP1 (Source: Uniprot.Org, NCBI)
Gene Name
CDCP1
Full Name
CUB domain-containing protein 1
Weight
92.932kDa
Alternative Names
CUB domain-containing protein 1; Membrane glycoprotein gp140; Subtractive immunization M plus HEp3-associated 135 kDa protein; SIMA135; Transmembrane and associated with src kinases; CD318; CDCP1; TRASK; UNQ2486/PRO5773 CDCP1 CD318, SIMA135, TRASK CUB domain containing protein 1 CUB domain-containing protein 1|membrane glycoprotein gp140|subtractive immunization M plus HEp3-associated 135 kDa protein|transmembrane and associated with src kinases
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CDCP1, check out the CDCP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CDCP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CDCP1 ELISA Kit PicoKine® (EK1668)
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No publications found for EK1668
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Human CDCP1 ELISA Kit PicoKine®
Question
Do you know the detection position of the antibodies in EK1668?
Verified customer
Asked: 2022-05-06
Answer
The antibodies in Human CDCP1 ELISA Kit PicoKine® (EK1668) were chosen to capture by the C-terminal domain of CDCP1 (aa 369-666) and detect by the N-terminal domain (aa 30-368). The immunogen sequence of the standard is aa 30-667.
Boster Scientific Support
Answered: 2022-05-06
Question
What is the “Human CDCP1 Standard” in EK1668? Is it a full-length CDCP1 or a fragment of CDCP1? What are the amino acid sequence?
Verified customer
Asked: 2020-10-16
Answer
For the Human CDCP1 ELISA Kit PicoKine™ (EK1668), the amino acid sequence of Human CDCP1 Standard is F30-T667.
Boster Scientific Support
Answered: 2020-10-18
Question
Which domains (CUB1, CUB2 or CUB3, or the range of amino acid sequence) do the capture and detector antibodies recognize for EK1668?
Verified customer
Asked: 2020-10-16
Answer
For the Human CDCP1 ELISA Kit PicoKine™ (EK1668), the immunogen sequence of capture and detector antibodies is A33-L666.
Boster Scientific Support
Answered: 2020-10-18
Question
Does EK1668 detect full-length, or cleaved, or both forms of CDCP1?
Verified customer
Asked: 2020-10-16
Answer
The Human CDCP1 ELISA Kit PicoKine™ (EK1668) detects CDCP1 aginst A33-L666.
Boster Scientific Support
Answered: 2020-10-18
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-08-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-26
Question
Q: can you suggest the dilution ratio of serum samples for detection of CDCP1 in Human serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-06-29
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CDCP1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-06-29
Question
Q: Can CDCP1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
K. Edwards
Verified customer
Asked: 2019-04-08
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-04-08
Question
Q: how can I thaw whole blood sample for CDCP1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CDCP1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-01-10
Question
Q: is it okay to use EDTA plasma as samples in Human CDCP1 Picokine® ELISA Kit (Catalog # EK1668)?
J. Evans
Verified customer
Asked: 2017-04-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CDCP1 causing degradation of its protein structure. CDCP1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CDCP1 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-04-04
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2016-01-31
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-01-31
Question
Q: What is the optimal O.D. value for CDCP1 ELISA kit? I performed your CDCP1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CDCP1 even though the O.D. values are not very high?
J. Wang
Verified customer
Asked: 2014-11-09
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CDCP1 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of CDCP1 that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2014-11-09
