Human CD7 ELISA Kit PicoKine®

CD7 ELISA kit for Human

Human CD7 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK2150
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human CD7 ELISA Kit PicoKine®

View all CD7 ELISA kits

SKU/Catalog Number

EK2150

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD7 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CD7 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2150)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: HEK293; Immunogen sequence: A26-P180

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK2150 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2150 is reactive to CD7 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2150 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CD7

CD7 (Cluster of Differentiation 7) is a protein that in humans is encoded by the CD7 gene. This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.

Kit Components

Catalog Number Description Quantity
EK2150-CAP Anti-Human CD7 Pre-coated 96-well strip microplate 1
EK2150-ST Human CD7 Standard 2 vials, 10 ng/tube
EK2150-DA Human CD7 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)015.631.262.51252505001,000
O.D.0.0150.0580.1250.2210.4740.8601.2542.256

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD7 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2216545126177426
Standard deviation1.2111.3932.021.411.1528.12
CV(%)5.5%6.9%7.1%5.1%6.3%6.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD7 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 122232528250.813.3%
Sample 21651571641631627.794.8%
Sample 345145846247346120.284.4%
*number of samples for each test n=16.

Gene/Protein Information For CD7 (Source: Uniprot.Org, NCBI)

Gene Name

CD7

Full Name

T-cell antigen CD7

Weight

25.409kDa

Alternative Names

CD7 antigen (p41); CD7 antigen; CD7 molecule; CD7; GP40T-cell surface antigen Leu-9; LEU-9; T-cell antigen CD7; T-cell leukemia antigen; Tp40; TP41; TP41p41 protein CD7 GP40, LEU-9, TP41, Tp40 CD7 molecule T-cell CD7|CD7 (p41)|T-cell leukemia |T-cell surface Leu-9|p41 protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD7, check out the CD7 Infographic

CD7 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EK2150

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Human CD7 ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Human CD7 ELISA Kit PicoKine®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Human CD7 ELISA Kit PicoKine®

$499
Backordered.

Lead time for this item is typically 2-3 weeks

Get A Quote
In stock
Order Product
EK2150
$499.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.