Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit

CCL21/6Ckine ELISA kit, 90-minute one-step protocol

Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL21/6Ckine/Exodus 2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: FEK0555
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Assay Time: 90 Minutes

How Quick Picokine(R) ELISA kits work: Get data in 90 minutes

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Product Name

Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit

See all CCL21/6Ckine products

SKU/Catalog Number

FEK0555

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL21/6Ckine/Exodus 2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0555)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: S24-P134

Sensitivity

<12 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of FEK0555 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0555 is reactive to CCL21 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK0555 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CCL21/6Ckine

Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

Kit Components

Catalog numberDescription QuantityVolume
FEK0555-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
EK0555-STHuman CCL21/6Ckine Standard 2 10 ng/tube
FEK0555-AHuman CCL21 Antibody A 1 3ml
FEK0555-BHuman CCL21 Antibody B 1 3ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)062.51252505001,0002,0004,000
O.D.0.0100.0620.1390.3130.4750.8021.5842.096

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)11782514121087941510
Standard deviation4.5630.5383.317.0234.9492.11
CV (%)3.9%3.7%5.9%6.5%4.4%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL21/6Ckine in ELISA kits from four different production batches/lots.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11171071021151106.065.5%
Sample 282578895290686815.621.8%
Sample 31412154214691397145559.664.1%
*number of samples for each test n=16.

Gene/Protein Information For CCL21 (Source: Uniprot.Org, NCBI)

Gene Name

CCL21

Full Name

C-C motif chemokine 21

Weight

14.646kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; CCL21; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4 CCL21 6Ckine, CKb9, ECL, SCYA21, SLC, TCA4 C-C motif chemokine ligand 21 C-C motif chemokine 21|Efficient Chemoattractant for Lymphocytes|beta chemokine exodus-2|chemokine (C-C motif) ligand 21|secondary lymphoid tissue chemokine|small inducible cytokine subfamily A (Cys-Cys), member 21

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCL21, check out the CCL21 Infographic

CCL21 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCL21: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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