Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit

Product Name

Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit

See all CCL21/6Ckine products

SKU/Catalog Number

FEK0555

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL21/6Ckine/Exodus 2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0555)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: S24-P134

Sensitivity

<12 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0555 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0555 is reactive to CCL21 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK0555 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CCL21/6Ckine

Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

Kit Components

Catalog number	Description	Quantity	Volume
FEK0555-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK0555-ST	Human CCL21/6Ckine Standard	2	10 ng/tube
FEK0555-A	Human CCL21 Antibody A	1	3ml
FEK0555-B	Human CCL21 Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)	0	62.5	125	250	500	1,000	2,000	4,000
O.D.	0.010	0.062	0.139	0.313	0.475	0.802	1.584	2.096

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CCL21/6Ckine/Exodus 2 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL21/6Ckine in ELISA kits from four different production batches/lots.

Gene/Protein Information For CCL21 (Source: Uniprot.Org, NCBI)

Alternative Names

6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; CCL21; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4 CCL21 6Ckine, CKb9, ECL, SCYA21, SLC, TCA4 C-C motif chemokine ligand 21 C-C motif chemokine 21|Efficient Chemoattractant for Lymphocytes|beta chemokine exodus-2|chemokine (C-C motif) ligand 21|secondary lymphoid tissue chemokine|small inducible cytokine subfamily A (Cys-Cys), member 21

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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