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Product Info Summary
| SKU: | EK1123 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CCL14/HCC-1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1123
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CCL14/HCC-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CCL14/HCC-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1123)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: T20-N93
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1123 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1123 is reactive to CCL14 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1123 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL14
Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1123-CAP | Anti-Human CCL14 Pre-coated 96-well strip microplate | 1 |
| EK1123-ST | Human CCL14 Standard | 2 vials, 10 ng/tube |
| EK1123-DA | Human CCL14 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentratio (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.007 | 0.023 | 0.069 | 0.161 | 0.359 | 0.670 | 1.288 | 1.955 |
Data Example Images
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Human CCL14/HCC-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCL14, Dilution ratio of 1:500, concentration in serum and plasma is around 60 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CCL14/HCC-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 37 | 238 | 445 | 40 | 227 | 446 |
| Standard deviation | 1.92 | 13.8 | 25.81 | 2.08 | 15.89 | 33.45 |
| CV (%) | 5.2% | 5.8% | 5.8% | 5.2% | 7% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL14 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 37 | 34 | 36 | 35 | 35 | 1.11 | 3.1% |
| Sample 2 | 238 | 250 | 251 | 216 | 238 | 14.09 | 5.9% |
| Sample 3 | 445 | 501 | 523 | 499 | 492 | 28.72 | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For CCL14 (Source: Uniprot.Org, NCBI)
Gene Name
CCL14
Full Name
C-C motif chemokine 14
Weight
10678 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 14;Chemokine CC-1/CC-3;HCC-1/HCC-3;HCC-1 (1-74);NCC-2;Small-inducible cytokine A14;HCC-1 (3-74);HCC-1 (4-74);HCC-1 (9-74);CCL14;NCC2, SCYA14; CCL14 CC-1, CC-3, CKB1, HCC-1, HCC-1(1-74), HCC-1/HCC-3, HCC-3, MCIF, NCC-2, NCC2, SCYA14, SCYL2, SY14 C-C motif chemokine ligand 14 C-C motif chemokine 14|chemokine (C-C motif) ligand 14|chemokine CC-1/CC-3|chemokine CC-3|hemofiltrate CC chemokine 1|new CC chemokine 2|small inducible cytokine subfamily A (Cys-Cys), member 14|small-inducible cytokine A14
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL14, check out the CCL14 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CCL14/HCC-1 ELISA Kit PicoKine® (EK1123)
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human CCL14/HCC-1 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
G. Mangal
Verified customer
Asked: 2020-12-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-12-20
Question
Q: if the enzyme conjugated CCL14 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL14 antigen?
H. Babu
Verified customer
Asked: 2020-11-16
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-16
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-11-05
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-11-05
Question
Q: how to thaw whole blood sample for CCL14 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL14 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-03-28
Question
Can EK1123 be used in urine sample?
Verified customer
Asked: 2019-03-11
Answer
For Human CCL14/HCC-1 ELISA Kit PicoKine™ (EK1123), we have not validated this kit with urine sample. In theory, the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest customers to run pilot experiments to decide the optimal sample dilution.
Boster Scientific Support
Answered: 2019-03-11
Question
Q: how to proceed with the analysis of ELISA data? I have performed CCL14 level in plasma.
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-03-06
Question
Q: can I use citrate plasma as samples in Human CCL14 Picokine® ELISA Kit (Catalog # EK1123)?
Verified Customer
Verified customer
Asked: 2018-06-03
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CCL14 causing disruption of its protein structure. CCL14 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CCL14 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-06-03
Question
Q: What is the optimal O.D. value for CCL14 ELISA kit? I used your CCL14 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CCL14 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2017-04-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL14 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CCL14 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2017-04-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
S. Surve
Verified customer
Asked: 2015-12-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-12-25
