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Product Info Summary
| SKU: | FEK0680 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human CA1/Carbonic anhydrase 1 PicoKine® Quick ELISA Kit
See all Carbonic Anhydrase I/CA1 products
SKU/Catalog Number
FEK0680
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CA1/Carbonic anhydrase 1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CA1/Carbonic anhydrase 1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human CA1/Carbonic anhydrase 1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0680)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A2-F261
Sensitivity
<50 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0680 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0680 is reactive to CA1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Application Guarantee
FEK0680 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Carbonic Anhydrase I/CA1
Carbonic anhydrase 1 is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participates in a variety of biologic processes like respiration, calcification, acid-base balance etc.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0680-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0680-ST | Human Carbonic Anhydrase I/CA1 Standard | 2 | 20 ng/tube |
| FEK0680-A | Human CA1 Antibody A | 1 | 3ml |
| FEK0680-B | Human CA1 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.149 | 0.282 | 0.395 | 0.594 | 0.945 | 1.467 | 1.983 | 2.303 |
Data Example Images
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Human CA1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CA1/Carbonic anhydrase 1 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 454 | 2602 | 9798 | 596 | 2668 | 9998 |
| Standard deviation | 19.06 | 148.31 | 627.07 | 30.39 | 120.06 | 559.88 |
| CV(%) | 4.2% | 5.7% | 6.4% | 5.1% | 4.5% | 5.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Carbonic Anhydrase I/CA1 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 454 | 513 | 503 | 485 | 488 | 17.56 | 3.6% |
| Sample 2 | 2602 | 2853 | 2812 | 2793 | 2765 | 88.48 | 3.2% |
| Sample 3 | 9798 | 9693 | 9697 | 8593 | 9445 | 500.58 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For CA1 (Source: Uniprot.Org, NCBI)
Gene Name
CA1
Full Name
Carbonic anhydrase 1
Weight
28.87kDa
Superfamily
alpha-carbonic anhydrase family
Alternative Names
CA1; CA-I; Car1; Carbonate dehydratase I; carbonic anhydrase 1; Carbonic anhydrase B; Carbonic Anhydrase I; carbonic anhydrase ICAB; carbonic dehydratase; EC 4.2.1.1 CA1 CA-I, CAB, Car1, HEL-S-11 carbonic anhydrase 1 carbonic anhydrase 1|carbonate dehydratase I|carbonic anhydrase B|carbonic anhydrase I|epididymis secretory protein Li 11|epididymis secretory sperm binding protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CA1, check out the CA1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CA1/Carbonic anhydrase 1 PicoKine® Quick ELISA Kit (FEK0680)
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