Anti-Carbonic Anhydrase I/CA1 Antibody Picoband®

Carbonic Anhydrase I/CA1 antibody

Boster Bio Anti-Carbonic Anhydrase I/CA1 Antibody catalog # PA1425. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PA1425
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Carbonic Anhydrase I/CA1 Antibody Picoband®

View all Carbonic Anhydrase I/CA1 Antibodies

SKU/Catalog Number

PA1425

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Carbonic Anhydrase I/CA1 Antibody catalog # PA1425. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Carbonic Anhydrase I/CA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1425)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Carbonic Anhydrase I, different from the mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1425 is reactive to CA1 in Human, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

29 kDa

Calculated molecular weight

28870 MW

Background of Carbonic Anhydrase I/CA1

Carbonic anhydrase 1 is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participate in a variety of biologic processes like respiration, calcification, acid-base balance etc.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1425 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Positive Control

WB: human HEL whole cell, rat spleen tissue

Validation Images & Assay Conditions

Gene/Protein Information For CA1 (Source: Uniprot.org, NCBI)

Gene Name

CA1

Full Name

Carbonic anhydrase 1

Weight

28870 MW

Superfamily

alpha-carbonic anhydrase family

Alternative Names

Carbonic anhydrase 1;4.2.1.1;Carbonate dehydratase I;Carbonic anhydrase B;CAB;Carbonic anhydrase I;CA-I;CA1; CA1 CA-I, CAB, Car1, HEL-S-11 carbonic anhydrase 1 carbonic anhydrase 1|carbonate dehydratase I|carbonic anhydrase B|carbonic anhydrase I|epididymis secretory protein Li 11|epididymis secretory sperm binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CA1, check out the CA1 Infographic

CA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Carbonic Anhydrase I/CA1 Antibody Picoband®

Question

Does PA1425 anti-Carbonic Anhydrase I/CA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

You can see on the product datasheet, PA1425 anti-Carbonic Anhydrase I/CA1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-04

Question

Will anti-Carbonic Anhydrase I/CA1 antibody PA1425 work for WB with pancreas spleen?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

According to the expression profile of pancreas spleen, CA1 is highly expressed in pancreas spleen. So, it is likely that anti-Carbonic Anhydrase I/CA1 antibody PA1425 will work for WB with pancreas spleen.

Boster Scientific Support

Answered: 2019-11-28

Question

Is this PA1425 anti-Carbonic Anhydrase I/CA1 antibody reactive to the isotypes of CA1?

A. Anderson

Verified customer

Asked: 2019-11-18

Answer

The immunogen of PA1425 anti-Carbonic Anhydrase I/CA1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Carbonic Anhydrase I(51-68aa SYNPATAKEIINVGHSFH), different from the mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-18

Question

Is a blocking peptide available for product anti-Carbonic Anhydrase I/CA1 antibody (PA1425)?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

We do provide the blocking peptide for product anti-Carbonic Anhydrase I/CA1 antibody (PA1425). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-11

Question

I was wanting to use your anti-Carbonic Anhydrase I/CA1 antibody for WB for rat pancreas spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat pancreas spleen identification?

Verified Customer

Verified customer

Asked: 2018-03-08

Answer

You can see on the product datasheet, PA1425 anti-Carbonic Anhydrase I/CA1 antibody has been tested for WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pancreas spleen in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-03-08

Question

Is there a BSA free version of anti-Carbonic Anhydrase I/CA1 antibody PA1425 available?

Verified Customer

Verified customer

Asked: 2017-06-12

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Carbonic Anhydrase I/CA1 antibody PA1425 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-06-12

Question

Will anti-Carbonic Anhydrase I/CA1 antibody PA1425 work on pig WB with liver?

S. Wu

Verified customer

Asked: 2013-04-23

Answer

Our lab technicians have not tested anti-Carbonic Anhydrase I/CA1 antibody PA1425 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Carbonic Anhydrase I/CA1 antibody PA1425 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-04-23

Order DetailsPrice
PA1425

100μg

$370
PA1425-10ug

10μg sample (liquid)

$99
PA1425-Biotin

100 μg Biotin conjugated

$570
PA1425-Cy3

100 μg Cy3 conjugated

$570
PA1425-Dylight488

100 μg Dylight488 conjugated

$570
PA1425-Dylight550

100 μg Dylight550 conjugated

$570
PA1425-Dylight594

100 μg Dylight594 conjugated

$570
PA1425-FITC

100 μg FITC conjugated

$570
PA1425-HRP

100 μg HRP conjugated

$570
PA1425-APC

100 μg APC conjugated

$670
PA1425-PE

100 μg PE conjugated

$670
PA1425-iFluor647

100 μg iFluor647 conjugated

$670
PA1425-carrier-free

Carrier Free

$370
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PA1425
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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