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Product Info Summary
| SKU: | FEK1560 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | bone tissue, cell culture supernatants, serum plasma (heparin, EDTA) and urine |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human BMP-9 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1560
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human BMP-9 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human GDF2 in bone tissue, cell culture supernatants, serum plasma (heparin, EDTA) and urine. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human BMP-9 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1560)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: S320-R429
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1560 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1560 is reactive to GDF2 in Human samples
Validated Sample Types
bone tissue, cell culture supernatants, serum plasma (heparin, EDTA) and urine
Application Guarantee
FEK1560 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BMP-9
Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP)-9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1560-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1560-ST | Human BMP-9 Standard | 2 | 10 ng/tube |
| FEK1560-A | Human GDF2 Antibody A | 1 | 3ml |
| FEK1560-B | Human GDF2 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.034 | 0.092 | 0.137 | 0.241 | 0.459 | 0.796 | 1.464 | 2.213 |
Data Example Images
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Human BMP-9 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human BMP-9 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 33 | 169 | 422 | 36 | 174 | 395 |
| Standard deviation | 1.49 | 8.45 | 24.48 | 2.56 | 12.01 | 21.73 |
| CV(%) | 4.5% | 5% | 5.8% | 7.1% | 6.9% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of BMP-9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 35 | 31 | 32 | 33 | 1.97 | 6% |
| Sample 2 | 169 | 181 | 184 | 174 | 177 | 9.74 | 5.5% |
| Sample 3 | 422 | 405 | 396 | 388 | 403 | 14.5 | 3.6% |
Protein Target Info & Infographic
Gene/Protein Information For GDF2 (Source: Uniprot.Org, NCBI)
Gene Name
GDF2
Full Name
Growth/differentiation factor 2
Weight
47.32kDa
Superfamily
TGF-beta family
Alternative Names
BMP9; BMP-9; BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2 GDF2 BMP-9, BMP9, HHT5 growth differentiation factor 2 growth/differentiation factor 2|bone morphogenetic protein 9
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GDF2, check out the GDF2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GDF2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human BMP-9 PicoKine® Quick ELISA Kit (FEK1560)
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