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Product Info Summary
| SKU: | EK1560 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | bone tissue, cell culture supernatants, serum plasma (heparin, EDTA) and urine. |
Product info
Product Name
Human BMP-9 ELISA Kit PicoKine®
SKU/Catalog Number
EK1560
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human BMP-9 ELISA Kit PicoKine® (96 Tests). Quantitate Human GDF2 in bone tissue, cell culture supernatants, serum plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human BMP-9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1560)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: S320-R429
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1560 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1560 is reactive to GDF2 in Human samples
Validated Sample Types
bone tissue, cell culture supernatants, serum plasma (heparin, EDTA) and urine.
Application Guarantee
EK1560 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BMP-9
Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP)-9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1560-CAP | Anti-Human GDF2 Pre-coated 96-well strip microplate | 1 |
| EK1560-ST | Human GDF2 Standard | 2 vials, 10 ng/tube |
| EK1560-DA | Human GDF2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.175 | 0.198 | 0.205 | 0.271 | 0.460 | 0.805 | 1.491 | 2.075 |
Data Example Images
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Human BMP-9 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect BMP-9, Dilution ratio of 1:1, concentration in serum and plasma is around 120 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human BMP-9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 35 | 186 | 405 | 34 | 178 | 376 |
| Standard deviation | 2.06 | 9.3 | 19.44 | 2.48 | 11.92 | 24.44 |
| CV (%) | 4.8%% | 5% | 4.8% | 7.3% | 6.7% | 6.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of BMP-9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 35 | 35 | 31 | 31 | 33 | 2 | 6% |
| Sample 2 | 186 | 184 | 182 | 187 | 184 | 1.92 | 1% |
| Sample 3 | 405 | 398 | 405 | 389 | 399 | 6.57 | 1.6% |
Protein Target Info & Infographic
Gene/Protein Information For GDF2 (Source: Uniprot.Org, NCBI)
Gene Name
GDF2
Full Name
Growth/differentiation factor 2
Weight
47.32kDa
Superfamily
TGF-beta family
Alternative Names
Growth/differentiation factor 2; GDF-2; Bone morphogenetic protein 9; BMP-9; GDF2; BMP9; GDF2 BMP-9, BMP9, HHT5 growth differentiation factor 2 growth/differentiation factor 2|bone morphogenetic protein 9
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GDF2, check out the GDF2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GDF2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human BMP-9 ELISA Kit PicoKine® (EK1560)
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human BMP-9 ELISA Kit PicoKine®
Question
Q: how to analyze ELISA data? I have obtained BMP-9 level in bone tissue.
M. Thomas
Verified customer
Asked: 2020-05-29
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-05-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-02-16
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-02-16
Question
Q: What is the optimal O.D. value for BMP-9 ELISA kit? I performed your BMP-9 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain BMP-9 even though the O.D. values are not very high?
T. Martinez
Verified customer
Asked: 2018-12-31
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BMP-9 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of BMP-9 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-12-31
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
H. Jones
Verified customer
Asked: 2018-12-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-12-10
Question
Q: can I use citrate plasma as samples in Human BMP-9 Picokine® ELISA Kit (Catalog # EK1560)?
A. Liu
Verified customer
Asked: 2018-11-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of BMP-9 causing disruption of its protein structure. BMP-9 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the BMP-9 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-11-12
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-08-26
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-08-26
Question
Q: which procedure should I follow in order to thaw whole blood sample for BMP-9 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BMP-9 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2017-12-14
Question
Q: if the enzyme conjugated BMP-9 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BMP-9 antigen?
Y. Perez
Verified customer
Asked: 2017-10-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-10-05
Question
Q: Can BMP-9 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2017-08-29
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2017-08-29
