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Product Info Summary
| SKU: | EK0310 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and bone tissue. |
Product info
Product Name
Human BMP-5 ELISA Kit PicoKine®
SKU/Catalog Number
EK0310
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human BMP-5 ELISA Kit PicoKine® (96 Tests). Quantitate Human BMP5 in cell culture supernatants, serum and bone tissue. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human BMP-5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0310)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A317-H454
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0310 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0310 is reactive to BMP5 in Human samples
Validated Sample Types
cell culture supernatants, serum and bone tissue.
Application Guarantee
EK0310 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BMP-5
Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on human chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0310-CAP | Anti-Human BMP5 Pre-coated 96-well strip microplate | 1 |
| EK0310-ST | Human BMP5 Standard | 2 vials, 10 ng/tube |
| EK0310-DA | Human BMP5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.003 | 0.101 | 0.143 | 0.275 | 0.437 | 0.889 | 1.421 | 2.026 |
Data Example Images
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Human BMP-5 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect BMP-5, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 156 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human BMP-5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 242 | 1573 | 4363 | 266 | 1683 | 4290 |
| Standard deviation | 16.45 | 69.21 | 314.13 | 20.74 | 82.46 | 347.49 |
| CV (%) | 6.8% | 4.4% | 7.2% | 7.8% | 4.9% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of BMP-5 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 242 | 228 | 246 | 239 | 238 | 6.68 | 2.8% |
| Sample 2 | 1573 | 1794 | 1863 | 1590 | 1705 | 126.02 | 7.3% |
| Sample 3 | 4363 | 4914 | 4157 | 4495 | 4482 | 276.84 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For BMP5 (Source: Uniprot.Org, NCBI)
Gene Name
BMP5
Full Name
Bone morphogenetic protein 5
Weight
51737 MW
Superfamily
TGF-beta family
Alternative Names
Bone morphogenetic protein 5;BMP-5;BMP5; BMP5 bone morphogenetic protein 5 bone morphogenetic protein 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BMP5, check out the BMP5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BMP5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human BMP-5 ELISA Kit PicoKine® (EK0310)
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Customer Q&As
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5 Customer Q&As for Human BMP-5 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for BMP-5 ELISA kit? I performed your BMP-5 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain BMP-5 even though the O.D. values are not very high?
D. Liu
Verified customer
Asked: 2020-08-20
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BMP-5 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of BMP-5 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-08-20
Question
Q: if the enzyme conjugated BMP-5 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BMP-5 antigen?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-03-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2017-02-19
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-02-19
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
R. Puri
Verified customer
Asked: 2016-01-26
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-01-26
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis BMP-5 using the EK0310 Human BMP-5 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-02-06
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2015-02-06
