Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit

Product Name

Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit

See all HGF products

SKU/Catalog Number

FEK0369

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human HGF in cell culture supernatants, serum and plasma (EDTA, citrate). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0369)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: Q32-R494 (alpha) & V495-S728 (beta)

Sensitivity

<5 pg/ml

Assay Range

125 pg/ml - 8,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0369 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0369 is reactive to HGF in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (EDTA, citrate).

Application Guarantee

FEK0369 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of HGF

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

Kit Components

Catalog number	Description	Quantity	Volume
FEK0369-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK0369-ST	Human HGF Standard	2	10 ng/tube
FEK0369-A	Human HGF Antibody A	1	3ml
FEK0369-B	Human HGF Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.014	0.081	0.116	0.217	0.357	0.631	1.261	2.033

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of HGF in ELISA kits from four different production batches/lots.

Gene/Protein Information For HGF (Source: Uniprot.Org, NCBI)

Alternative Names

deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A HGF DFNB39, F-TCFB, HPTA, SF, HGF hepatocyte growth factor hepatocyte growth factor|fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor (hepapoietin A; scatter factor)|hepatopoietin-A|lung fibroblast-derived mitogen

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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