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Product Info Summary
| SKU: | LS009162 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of diacylglycerol kinase, iota (DGKI) |
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Product info
Product Name
DGKI (NM_004717) Human Over-expression Lysate
SKU/Catalog Number
LS009162
Size
100 µg
Description
Transient overexpression lysate of diacylglycerol kinase, iota (DGKI)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
DGKI (NM_004717) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009162)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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DGKI Human Over-expression Lysates NM_004717.
Western blot validation of overexpression lysate (LS009162) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with DGKI (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for DGKI (Source: Uniprot.org, NCBI)
Gene Name
DGKI
Full Name
Diacylglycerol kinase iota
Weight
116.997kDa
Superfamily
eukaryotic diacylglycerol kinase family
Alternative Names
DAG kinase iota; DGKI; DGKiota; DGK-iota; diacylglycerol kinase iota; diacylglycerol kinase, iota; Diglyceride kinase iota; EC 2.7.1.107 DGKI DGK-IOTA diacylglycerol kinase iota diacylglycerol kinase iota|DAG kinase iota|diglyceride kinase iota
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on DGKI, check out the DGKI Infographic
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In this infographic, you will see the following information for DGKI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For DGKI (NM_004717) Human Over-expression Lysate (LS009162)
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No publications found for LS009162
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