Product Info Summary
SKU: | A09710-1 |
---|---|
Size: | 25uL |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Cybr CYTIP Antibody
SKU/Catalog Number
A09710-1
Size
25uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Cybr CYTIP Antibody (Catalog # A09710-1). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. Expiration date is three (3) months from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Cybr CYTIP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09710-1)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the carboxy terminal portion of mouse Cybr.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09710-1 is reactive to CYTIP in Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
45983 MW
Background of CYTIP
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Cybr (Pscdbp) is a new scaffold protein with significant homology to Tamalin. It contains a PDZ binding domain and a coiled-coil domain. Cybr is highly expressed in the immune system, and its expression seems to be common to nearly all immune cell populations (T cells, B cells, NK Macrophages, dendritic cells). Other organs/tissues in which Cybr is expressed include lung, kidney, intestine and testes. Cybr may play an important in vivo role in cell migration, especially when modulated by pro-inflammatory cytokines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09710-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IHC: User optimized
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CYTIP using anti-CYTIP antibody (A09710-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYTIP antigen affinity purified polyclonal antibody (Catalog # A09710-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CYTIP.
Protein Target Info & Infographic
Gene/Protein Information For CYTIP (Source: Uniprot.org, NCBI)
Gene Name
CYTIP
Full Name
Cytohesin-interacting protein
Weight
45983 MW
Alternative Names
Cybr, Cytohesin-interacting protein, Cytohesin-binding protein HE, Cbp HE, Pleckstrin homology Sec7 and coiled-coil domains-binding protein, Pscdbp, Cytip CYTIP B3-1, CASP, CYBR, CYTHIP, HE, PSCDBP cytohesin 1 interacting protein cytohesin-interacting protein|cbp HE|cytohesin binder and regulator|cytohesin binding protein HE|cytohesin-associated scaffolding protein|pleckstrin homology Sec7 and coiled-coil domains-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYTIP, check out the CYTIP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYTIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cybr CYTIP Antibody (A09710-1)
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Customer Q&As
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3 Customer Q&As for Anti-Cybr CYTIP Antibody
Question
Does anti-Cybr antibody A09710-1 work for WB with blood?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
According to the expression profile of blood, CYTIP is highly expressed in blood. So, it is likely that anti-Cybr antibody A09710-1 will work for WB with blood.
Boster Scientific Support
Answered: 2019-05-21
Question
Here is the WB image, lot number and protocol we used for blood using anti-Cybr antibody A09710-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-13
Question
We are currently using anti-Cybr antibody A09710-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
M. Krishna
Verified customer
Asked: 2015-11-30
Answer
The anti-Cybr antibody (A09710-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-30