Coronin 1a (CORO1A) (NM_007074) Human Over-expression Lysate

Lysate of Coronin-1a over-expression cell lines, WB control

Transient overexpression lysate of coronin, actin binding protein, 1A (CORO1A)

Product Info Summary

SKU: LS011151
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of coronin, actin binding protein, 1A (CORO1A)

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Product Name

Coronin 1a (CORO1A) (NM_007074) Human Over-expression Lysate

See all Coronin-1a products

SKU/Catalog Number

LS011151

Size

100 µg

Description

Transient overexpression lysate of coronin, actin binding protein, 1A (CORO1A)

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

Coronin 1a (CORO1A) (NM_007074) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS011151)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for CORO1A (Source: Uniprot.org, NCBI)

Gene Name

CORO1A

Full Name

Coronin-1A

Weight

51.026kDa

Superfamily

WD repeat coronin family

Alternative Names

CLIPINA; clipin-A; CORO1; coronin, actin binding protein, 1A; coronin, actin-binding protein, 1A; coronin-1; coronin-1A; Coronin-like protein A; Coronin-like protein p57; FLJ41407; HCORO1; MGC117380; p57; TACOCLABP; Tryptophan aspartate-containing coat protein CORO1A CLABP, CLIPINA, HCORO1, IMD8, TACO, p57 coronin 1A coronin-1A|clipin-A|coronin, actin binding protein, 1A|coronin-1|coronin-like protein A|coronin-like protein p57|tryptophan aspartate-containing coat protein

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on CORO1A, check out the CORO1A Infographic

CORO1A infographic

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In this infographic, you will see the following information for CORO1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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