CD98 (SLC3A2) (NM_001012662) Human Over-expression Lysate

Product Name

CD98 (SLC3A2) (NM_001012662) Human Over-expression Lysate

See all CD98 products

SKU/Catalog Number

LS006520

Size

100 µg

Description

Transient overexpression lysate of solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 (SLC3A2), transcript variant 2

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

CD98 (SLC3A2) (NM_001012662) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006520)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SLC3A2 (Source: Uniprot.org, NCBI)

Alternative Names

4F2; 4F2hc; 4T2HC; antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC; CD98 antigen; CD98 heavy chain; CD98; CD98HC; CH98hc; FRP-1; heavy chain; Lymphocyte activation antigen 4F2 large subunit; MDU1antigen defined by monoclonal 4F2, heavy chain; monoclonal 44D7; NACAE4F2 cell-surface antigen heavy chain; SLC3A2; solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen SLC3A2 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE solute carrier family 3 member 2 4F2 cell-surface heavy chain|CD98 heavy chain| defined by 4F2, heavy chain| by antibodies 4F2, TRA1.10, TROP4, and T43|lymphocyte activation 4F2 large subunit| 44D7|solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2|solute carrier family 3 (amino acid transporter heavy chain), member 2

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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